Table I

Polypeptides deregulated in invasive TCCs

IdentityMolecular mass (kDa)pIMethod of identificationFunctionAltered expression incidenceaAntibodies and specificitybImmunofluorescence in fixed cryostat sections
Up-regulated polypeptides
EF 1-γ33.04.3Mass spectrometry, comparison with the keratinocyte 2D-PAGE databaseProtein synthesis9/15 (60%)
hsp2825.56.0Mass spectrometry, comparison with the keratinocyte 2D-PAGE databaseStress response: heat shock, cell differentiation19/24 (79%)Rabbit polyclonalc SpecificNegative
CK1843.65.3Mass spectrometry, 2D-PAGE immunoblottingCytoskeleton assembly13/23 (57%)Monoclonald SpecificPositive
CK2045.75.6Mass spectrometry, 2D-PAGE immunoblottingCytoskeleton assembly6/18 (33%)Monoclonald SpecificPositive
PCNA35.84.4Mass spectrometry, 2D-PAGE immunoblottingCell cycle and differentiation16/21 (76%)Rabbit polyclonalc SpecificNegative
Proteasome ζ28.24.4Mass spectrometryProtein turnover11/21 (52%)
MRP1411.25.6Mass spectrometry, comparison with the keratinocyte 2D-PAGE databaseCalcium-modulated metabolism: cell migration14/17 (82%)Rabbit polyclonalc SpecificNegative
Down-regulated polypeptides
A-FABP11.76.2Mass spectrometry, 2D-PAGE immunoblottingCell cycle and differentiation: fatty acid transport25/25 (100%)Rabbit polyclonalc SpecificNegative
Annexin V35.54.8Mass spectrometry, 2D-PAGE immunoblottingCalcium-modulated metabolism: Ca and phospholipid-binding protein17/23 (74%)Rabbit polyclonale SpecificNegative
High affinity bile-binding protein (DD2)36.77.5Edman degradationMember of oxidoreductase gene family: multifunctional protein, involved in bile acid transport9/17 (53%)
CK557.87.82D-PAGE immunoblottingCytoskeleton assembly12/17 (71%)Rabbit polyclonalc Slight cross-reactivity with CKs 7 and 8Positive
CK1352.24.82D-PAGE immunoblottingCytoskeleton assembly20/26 (77%)Rabbit polyclonalc SpecificPositive
Lactate dehydrogenase H chain35.55.7Mass spectrometryEnergy metabolism: mitochondrial respiration17/22 (77%)
Maspin38.25.8Mass spectrometryCell cycle and differentiation: tumor supression17/22 (77%)Rabbit polyclonalc SpecificNegative
PAI-241.35.5Mass spectrometryMember of serpine protease-inhibitor family: extracellular proteolysis16/25 (64%)Rabbit polyclonale SpecificNegative
PA-FABP12.46.3Mass spectrometry 2D-PAGE immunoblottingCell cycle and differentiation: fatty acid transport22/23 (96%)Rabbit polyclonalc SpecificNegative
PGM31.06.8Mass spectrometryEnergy metabolism20/22 (91%)
PnPH31.96.4Mass spectrometryCell cycle and differentiation: DNA metabolism19/22 (86%)
Tropomyosin33.74.4Mass spectrometry, comparison with the keratinocyte 2D-PAGE databaseActin filament assembly15/21 (71%)
14-3-3 ς30.04.4Mass spectrometry, comparison with the keratinocyte 2D-PAGE databaseCell cycle and differentiation: mitogenic signal transduction17/20 (85%)Rabbit polyclonalc Cross-reactivity with other family membersNegative
Unknownf45.65.69/17 (53%)
Unknownf31.76.911/16 (69%)
  • a Based on the 2D gel analysis of 30 invasive TCCs (Gr III, T2-4) labeled with [35S]methionine. In several cases the entire gel image could not be used for analysis because of focusing problems in different areas of the gels. Only major proteins exhibiting more than a 2-fold change are listed.

  • b Determined by 2D gel Western blotting.

  • c Prepared in our laboratory.

  • d Available commercially.

  • e Obtained from colleagues.

  • f Unknown in the TCC 2D-PAGE database.