Table I

Phosphopeptides isolated from Net1N phosphorylated in vitro by Cdc5

Phosphorylated amino acids that were definitively identified are in bold. Residues that could potentially be phosphorylated are in italics.

PeptideSequenceAmino acidsMr
1TNMAQSAGDApSLQYANLR38–551989.9
2SANVpSFTPSYFNQSR56–701783.8
3SANVpSFTPSYFNQSR + 2P56–701863.8
4pSANVpSFTPpSYFNQSR56–701943.8
5VILKNEIDLDDSAPVpSLYK137–1552211.1
6SKLNNGSPQSVQPQQQIPSSSGVLR + 1P160–1842715.4
7LNNGSPQSVQPQQQIPSSSGVLR + 1P162–1842500.2
8KRPPpTGTTTTTTIR188–2011609.9
9RPPTGTTTTTTIR + 1P189–2011481.8
10IVSNNSDDEDEDIGERpSFLPPPTQPQSPPIR226–2563528.6
11IVSNNpSDDEDEDIGERpSFLPPPTQPQSPPIR226–2563608.6
12ISpSGIDAGKK257–2661054.5
13IKpSSIVEEDIVSR267–2791553.8
14SSIVEEDIVSRpSATVDPDKpTKQQR269–2922847.4
15SATVDPDKpTKQQR280–2921552.8