Table II

Net1N mutants and their phenotypes

MutantNet1N mutant allelesa-Fold defect
1S60 S64 S242 S335<3
2S60 S64 S242 S483
3S60 S64 S242 S335 S48 (5m)3
4S60 S64 S242 S335 S48 S269 S2703
5S60 S64 S242 S335 S48 S269 S270 S2073
6S60 S64 S242 S335 S48 S269 S270 S207 S152 S563
7S60 S64 S242 S335 S48 S152 S156 S2803
8S60 S64 S242 S335 S48 S269 S270 S207 S152 S56 S156 S2803
9S60 S64 S242 S335 S48 S269 S270 S207 S152 S56 S156 S280 S202 T62 S178 S179 S1803
10S60 S64 S242 S335 S48 S269 S270 S207 S152 S56 S156 S280 S202 T62 S178 S179 S180 T192 T288 (19m)3
11S60 S64 S242 S335 S48 S269 S270 S207 S152 S56 S156 S280 S202 T62 S178 S179 S180 T192 T288 S12 T16 T26 S30 S3110
12S60 S64 S242 S335 S48 S12 T16 T26 S30 S3110
13S60 S64 S242 S48 S12 T16 T26 S30 S3110
14S60 S64 S242 S335 S48 T26<3
15S60 S64 S242 S335 S48 T26 S30 S3110–30
16S60 S64 S242 S335 S48 T26 S30 S31 T38 T28210
17S60 S64 S242 S335 S48 T26 S30 S31 S223 S224 S301 T30210
18S60 S64 S242 S335 S48 S269 S270 S207 S152 S56 S156 S280 S202 T62 S178 S179 S180 T192 T288 T16 T26 S30 S31 S223 S224 S228 S231 S301 T30210b
19S60 S64 S242 S335 S48 S30 S31 (7m)30
20S60 S64 S242 S335 S48 S30 S31 T16 S223 S224 S228 S231 S295 S301 T302 T194 T195 T196 T19730
  • a All indicated amino acids were mutated to alanine.

  • b High background level of release when kinase was not included in the reaction, suggesting weakened interaction between the mutant Net1N and Cdc14.