Table III

Phosphopeptides isolated from Net1N-19m phosphorylated in vitro by Cdc5

Phosphorylated amino acids that were definitively identified are in bold. Residues that could potentially be phosphorylated are in italics.

SequenceAmino acidsMr
LQVVLVPPSLQATMPIQFGYGPTIAESSQLLPNRTNMAQSAGDAALQYANLR + 2P4–555699.9
LQVVLVPPSLQATMPIQFGYGPTIAESSQLLPNRTNMAQSAGDAALQYANLR + 3P4–555779.9
LQVVLVPPSLQATMPIQFGYGPpTIAESSQLLPNRpTNMAQpSAGDAALQYANLR + 4P4–555859.9
LQVVLVPPSLQATMPIQFGYGPTIAESSQLLPNRTNMAQSAGDAALQYANLR + 5P4–555939.9
KRPPAGTTTTTTIR + 1P188–2011579.8
KRPPAGTTTTTTIR + 2P188–2011659.8
VSTPLAR + 1P210–216822.4
QIYPPPSSK + 1P217–2251095.5
QIYPPPSSKIVSNNSDDEDEDIGER + 1P217–2412883.3
QIYPPPSSKIVSNNSDDEDEDIGER + 2P217–2412963.3
IVSNNpSDDEDEDIGER226–2411885.8
AAIVEEDIVpSR269–2791280.6
AApTVDPDKAK280–2891094.5
LLSGTPIMSTMTPNR + 1P293–3071697.8
LLSGTPIMSTMTPNR + 2P293–3071777.8
VTLTGQR + 1P308–314853.4
VVSEHAHKNELVFSASASSSAFANGGTGGSIDVEHHHHHH + 1P315–3544262.9
VVSEHAHKNELVFSASASSSAFANGGTGGSIDVEHHHHHH + 2P315–3544342.9
VVSEHAHKNELVFSASASSSAFANGGTGGSIDVEHHHHHH + 3P315–3544422.9
VVSEHAHKNELVFSASASSSAFANGGTGGSIDVEHHHHHH + 4P315–3544502.9