Table I

Identification by LC/MS/MS of human axonemal proteins separated by 2-D PAGE

SpotDescriptionAccession No.No. of peptides
1A1cDNA FLJ23305 FISQ9H5M38
2A2hb08b03.×1 cDNA CloneAW4737701
3A2P97 ATPase-related sequenceCAB4152512
4A2KIAA0356 ProteinQ9Y4G21
5A3TER ATPaseP464621
6A5Heat shock proteinHS703
7A5Heat shock cognate 70-kDa protein3HSC2
8A5602350570F1 cDNA CloneBG1223871
9A5Stratagene fetal retinalAA1768901
10A6Heat shock protein 70P081074
11A6HS7C Mouse heat shock cognate 71-kDaP081094
12A6TERA human endoplasmic reticulum ATPaseP550721
13A8Testicular microtubules-related protein Tektin 3AAK153405
14A9Unnamed protein productBAA920775
15A10α-TubulinAAH049494
16A11β-TubulinQ604556
17B1NEB proteinAF0226501
18B1β-TubulinAF0830311
19B2Canine TektinQ9TTW38
20B4Unnamed protein productBAB714023
21B4β-TubulinQ604555
22B4Reticulocyte-binding proteinQ007981
23B4KIAA0356 ProteinQ9Y4G21
24B4SPAMQ540641
25B4α-TubulinQ9DFT41
26B4Similar to Tektin-3AAH217161
27B4Unnamed protein productBAB714021
28B5α-TubulinQ9GLW62
29B5Similar to Tektin-3AAH217162
30B6Similar to Tektin-3AAH217162
31B6β-TubulinQ9JJY62
32B7H-Tektin-TQ9UIF35
33B8H-Tektin-TO606386
34B9Similar to Tektin-3AAH217161
35B9H-Tektin-TQ9UIF31
36B9CGI-53 ProteinAF1518113
37B10β-ActinQ612762
38B11β-ActinX037653
39B12CGI-53 proteinAF1518113
40B12β-TubulinP043501
41B12H-Tektin-TQ9UIF31
42B12Similar to Tektin-3AAH217161
43C1β-TubulinQ604555
44C2β-TubulinQ604553
45C2Similar to Tektin-3AAH217161
46C4α-TubulinQ081141
47C5β-TubulinQ604559
48C6α-TubulinQ436051
49C6Novel protein isoform 1Q9H1X02
50C9Similar to Tektin-3AAH217161
51C11Heat shock protein J2O751907
52C11β-TubulinQ9JJY62
53C11AKAP-associated sperm proteinAAG595871
54C12HU EST cDNABG4232151
55C12HU EST cDNABF9654222
56C12H-Tektin-TQ9UIF37
57D3Human nucleoside diphosphate kinase homologP565975
58D4EIF-3-βQ133471
59D5Similar to myeloid leukemia factorAAH039752
60D6Heat shock protein J2AAH024465
61D8β-TubulinP092063
62D9β-TubulinP092064
63D10β-TubulinP092064
64D1014-3-3 Protein εU538822
65D10KIAA0477 ProteinO750651
66D11Similar to Riken proteinBC0073742
67D12Similar to Riken proteinBC0073742
68E3Unknown proteinAAH045224
69E5Putative GTP-binding proteinAAH005663
70E6CG11245 ProteinQ9VYN11
71E8Mus Musculus male testis cDNAAK0056091
72E9Predicted proteinXP_0884901
73E10c-Myc-binding proteinQ994174
74E12β-TubulinQ9JJY61
75F2Mus Musculus male testis cDNAAK0156971
76F3Fusion geneAAA999972
77F4Fusion geneAAA999972
78F5Fusion geneAAA999973
79F8Mus Musculus male testis cDNAAK0057393
80F8LEPS BACSU signal peptidaseP286281
81F12AKAP-associated sperm proteinAAG595872
82F12α-TubulinQ063312
83F12Phenylalanyl-tRNA synthetase β-chainQ9ZDB41