Table II

Combined results of four different proteomic analyses of human ciliary axonemes

DescriptionAccession No.2-D gel1-D gelDirect LC/MS/MSMulti-dimensional LC/MS/MS
1α-TubulinMultipleXXXX
2β-TubulinMultipleXXXX
370-kDa Heat shock protein(s)MultipleXXXX
4Heat shock protein(s) J2MultipleXXXX
5ActinMultipleXXXX
614-3-3 ProteinsMultipleXXX
7Stratagene fetal retinalAA1768901
8Fusion geneAAA99997321
9AKAP-associated sperm proteinAAG5958721
10Putative GTP-binding proteinAAH0056632
11Similar to myeloid leukemia factorAAH039752
12Unknown proteinAAH045224
13Testicular microtubules-related protein Tektin 3AAK153405
14Kinesin-like protein 38B (NEB protein)AF0226501
15CGI-53 ProteinAF15181151
16Mus Musculus male testis cDNAAK0056091
17Mus Musculus male testis cDNAAK0057393
18Mus Musculus male testis cDNAAK0156971
19hb08b03.×1 cDNA cloneAW4737701
20Unnamed protein productBAB714024
21Similar to Riken cDNA 4933428D01BC00737421
22P97 ATPase-related sequenceCAB4152512
23H-Tektin-TMultipleXX
24KIAA0477 ProteinO750651
25LEPS BACSU signal peptidaseP2862811
26Tera human endoplasmic reticulum ATPaseP5507211
27Human nucleoside diphosphate kinase homolog (testis-specific)P565975511
28Reticulocyte-binding proteinQ007981
29EIF-3-βQ133471
30SPAMQ540641
31c-Myc-binding proteinQ9941741
32Novel protein isoform 1Q9H1X02
33Unnamed protein productBAA9207752
34CG11245 ProteinQ9VYN111
35KIAA0356 ProteinQ9Y4G21
36Phenylalanyl-tRNA synthetase β-chainQ9ZDB41
37cDNA FLJ23305 FISQ9H5M381
38Predicted proteinXP_0884901
39Annexin 1MultipleXXX
401CMI_A Protein inhibitor of neuronal nitric oxide PDB1CMIA2
41Galectin-73GALA1
42CLP-like protein fragmentAAF643021
43HU ciliary dynein heavy chain 9AAF6900432
44AAF97774 J4RAAF977741
45IC1 HomologAAG2849751
46Putative glutathione S-transferase OSGSTU4AAG324711
47HU DNA sequence clone RP1-261G23AL1361316
48cDNA DKFZp434N2435AL1368582
49cDNA FLJ20753 FISBAA913651
50cDNA FLJ10466 FISBAA91628121
51KIAA1374 Protein (fragment)BAA926122
52KIAA1430 Protein (fragment)BAA926681
53LUNX proteinBAA936331
54Unnamed protein productBAB016029
55Genomic DNA chromosome 5, TAC clone:K3K7BAB087431
56Putative cysteine proteinaseBAB170961
57Similar to part of several eg. ACDS_MEGEL Q06319 Acyl-CoA dehydrogenaseCAB096281
58CAB70790 Hypothetical 32.7-kDa proteinCAB707903
59CAB71195 DJ111C20.1 (similar to chlamydomonas radial spoke protein)CAB711952
60CAB92087DJ534K7.2 (novel protein)CAB9208731
61DJ412I7.1 (similar to radial spokehead protein) (fragment)CAC007714
62Hypothetical 37.1-kDa proteinCAC0952612
63Intermediate dynein chainCAC174641
64Canis β-galactosides-binding lectinL234292
65HU chloride intracellular channel protein 1O002992
66Probable dolichyl-phosphate-mannose-protein mannosyltransferaseO138981
67Axonemal dynein light chainO1464511
68Nephrocystin (fragment)O148372
69Mouse carnitine deficiency-associated (CDV-1) proteinO355942
70Tegument protein/FGARATO419771
71Probable dynein protein (fragment)O43352712
72Hypothetical 16.9-kDa proteinO581431
73O60780 DJ206D15.3O6078011
74Hypothetical 20.4-kDa proteinO608973
75Hypothetical 50.3-kDa proteinO698231
76hsp89-α-Δ-NO753221
77Dega-like proteinO881611
78Type II peroxiredoxin 1O883761
79Hypothetical 363.6-kDa proteinO972781
80PHNA-like proteinO9PIU41
81Yeast threonine dehydratase precursorP009271
82TERM_BPPH2 DNA terminal proteinP036811
83HU heat shock 27-kDa proteinP047923
84Human annexin II (lipocortin II) (calpactin I heavy chain) (chromobindin 8)Multiple9
85Human calpain 1, large (catalytic) subunitP073841
86Annexin VP087588
87HU glutathione S-transferase PP092111
88HU annexin 4P095255
89Ezrin (P81) (cytovillin)P1531142
90Human annexin VII (synexin)P200735
91cAMP-dependent protein kinase, α-catalytic subunitP277914
92Mouse murinoglobulin 1 precursor (MUG1)P286651
93Human squamous cell carcinoma antigen 1 (SCCA-1)P295081
94Human Serine/Threonine protein phosphatase 2A, 65-kDa regulatory subunit A, α-IsoformP3015312
95SORC Human sorcinP306262
96Human transformation-sensitive protein IEF SSP 3521P319481
97Guanine nucleotide-binding protein G(1), α-2 subunit (adenylate cyclase-inhibiting G α-proteinP384001
98Human caltractinP4120811
99MYCMY RECA proteinP424661
100Kinesin-like protein FLA10 (KHP1 protein)P468691
101Casein kinase I, α-IsoformP487293
102HU oxygen-regulated protein 1 (retinitis pigmentosa RP1 protein)P5671511
103Glucose-regulated protein/BIP precursorQ127521
104Nascent polypeptide-associated complex α-subunitQ137651
105CAYP Human calcyphosineQ139385
106Human Nedd5 protein homolog (KIAA0158)Q1501911
107HU sperm surface protein Sp17Q15506111
108Human 110-kDa cell membrane glycoproteinQ161861
109ORF2 5′ to PD-ECGF/TP proteinQ161931
110DY13_ANTCR dynein intermediate chain 3, ciliaryQ169601
111C07B5.4 proteinQ177771
112DYL_1CAEEL probable dynein light chain 1 cytoplasmicQ227992
113Axonemal dynein light chain p33Q266301
114Dynein heavy chain isotype 3AQ2780321
115Dynein heavy chain isotype 7AQ278112
116Q52643 ORF2Q526431
117Cytoplasmic dynein 3 heavy chain (fragment)Q928161
118B7 proteinQ929774
119β-Dynein heavy chainQ9MBF81
120DJ622L5.6 (novel protein) (fragment)Q9NU802
121cDNA FLJ10466 FISQ9NVW614
122Related to TOM1 proteinQ9P4Z11
123F22D16.14 proteinQ9SRX91
124Hypothetical 35.9-kDa protein (fragment)Q9UF431
125Hypothetical 27.1-kDa protein (fragment)Q9UF455
126Hypothetical 34.8-kDa protein (fragment)Q9UFG851
127Hypothetical 186.7-kDa protein (fragment)Q9UG012
128Hypothetical 36.8-kDa protein (fragment)Q9UGA21
129Hypothetical 19.3-kDa proteinQ9UGQ05
130Rhabdoid tumor deletion region protein 1Q9UHP67
131Dynein intermediate chain DNAI1Q9UI4613
132Novel protein similar to WORM E04F6.2 (fragment)Q9UJF84
133CDV-1 proteinQ9UNY82
134KIAA1023 protein (fragment)Q9UPX71
135Q9VGA0 CG10091 proteinQ9VGA01
136CG9492 proteinQ9VH971
137KIAA0944 protein (fragment)Q9Y2F331
138Axonemal dynein heavy chain protein DNAHQ9Y3I61
139Hypothetical 18.0-kDa proteinQ9Y4131
140Hypothetical 18.4-kDa proteinQ9Y4P92
141NM23-H7Q9Y5B89
142Brain-specific proteinQ9Y6H01
143MAP kinase phosphatase-like protein MK-STYXQ9Y6J81
144Factor C protein precursorQ9Z4K01
145Mus musculus AC39/physophilin mRNAU215492
146Hypothetical 35.9-kDa protein (fragment)X032841
147Homo sapiens laminin-binding proteinX611562
148Nasopharyngeal epithelium-specific protein 1Q9UL16224
149hspc162/bithoraxoidAAF2912611
150Growth hormone receptorAAF671701
151Axonemal dynein heavy chainCAB947566
152Y17D7C.1 proteinO624071
153WUGSC:H_RG252K19.1 protein (fragment) (dynein β-chain, ciliary by FASTA)O957051
154Predicted integral membrane proteinO961201
155Human calgizzarin (S100C protein)P319491
156TG737Q130991
157HU dynein light chain 1, cytoplasmicQ157011
158Dynein γ-chain, flagellar outer armQ395751
159Dynein intermediate chain DNAI1Q9UI451
160Putative movement proteinQ9XG721
161Unknown proteinAAH030241
162Mouse GLTSAAH051521
163DPY-30-like proteinAAK006401
164MHS4R2AAK135881
165Dynein-associated proteinAAK187111
166Radial spoke protein 3AAK264321
167PtnD proteinAAK029161
168Predicted proteinAE0063511
169Mus Musculus male testis cDNAAK0170561
170KIAA1770 proteinBAB218611
171Dynein light chain, Hp28CAC360871
172Novel proteinCAC373031
173KIAA0357 proteinO150641
174Hypothetical proteinO171211
175ARCFU putative translation initiation factorO282141
176Pyruvate dehydrogenaseO431061
177PP32R1.GPMO434231
178T27E7.6 proteinO458591
179Hypothetical 99.5-kDa proteinO677961
180KIAA0643 proteinO751381
181Sperm flagellar protein, SPAG6O756021
182Hypothetical protein 56.0 kDaO840541
183Growth arrest proteinO959951
184Pyruvate dehydrogenaseP105151
185StomatinP271052
186PP11_DROME Serine/Threonine protein phosphataseP484611
187FLGK_BUCAI flagellar hook-associated proteinP574281
188Phenylalanyl-tRNA synthetase α-chainP942821
189Dynein light chainQ157631
190Hypothetical protein 11.7 kDaQ515721
191Hypothetical protein 133.1 kDaQ941851
192Kinesin-like calmodulin-binding proteinQ9FQL71
193Hypothetical 55.2-kDa proteinQ9H0C01
194Hypothetical protein 76.7 kDaQ9H0K55
195DyneinQ9H1795
196Novel proteinQ9H1V91
197Novel proteinQ9H1X15
198FLJ22843 FISQ9H5X52
199FLJ22601 FISQ9H6531
200cDNA FLJ14117Q9H7X71
201cDNA FLJ13946Q9H8491
202KIAA1640 proteinQ9HCD22
203KIAA1603 proteinQ9HCG92
204Similar to radial spokehead proteinQ9NQ102
205C11ORF16 proteinQ9NQ321
206Hypothetical protein 34.3 kDaQ9NQC82
207Hypothetical protein 47.7 kDaQ9NTC01
208Novel Serine/Threonine protein kinaseQ9NUH71
209Dynein heavy chainQ9NYC96
210Hypothetical protein 77.2 kDaQ9P1V81
211KIAA1374 proteinQ9P2H31
212CG6892 proteinQ9VC381
213Hypothetical protein 66.0 kDaQ9Y4Q11
214cDNA FLJ10466QPNVW61