Table IV

Predicted organelle targeting by Web-based programs

Protein aSpecies bLocalization cChloroPTargetPPSORT dPredotarMitoProt
PsaEIVAtSYCO:V:W:E/+P0.95
PsaEIIAtSYCW:E:O:X/?P0.95
PsaESoSYCS:M:T:L/?M0.80
PsaDIVAtSYCN:S:M:X/?P0.96
PsaDSoSYCN:S:M:T/?P0.94
Lhcb1SoT+NCX:S:M:T/?N0.45
PetCPsT+YCP:T:S:L/+P0.36
Lhcb1*4PsT+YCS:X:T:L/?P0.01
Lhcb1*2PsT+YCS:T:L:X/+N0.81
Lhcb4VAtT+YCI:X:R:M/?P0.99
Lhcb5IVAtT+YCT:I:P:X/?P0.68
PsbSIAtT+YCT:P:G:I/−N0.92
Lhca3IAtT+YCS:M:T:X/+P0.96
Lhcb1–7NtT+YCS:M:T:X/+N0.86
Lhcb1–40NtT+YCS:T:L:X/+P0.70
Lhcb1II2AtT+YCS:X:T:L/+N0.85
Lhcb1–21NtT+YCS:X:T:M/+N0.86
Lhcb2IIαAtT+YCT:X:S:L/+P0.86
Lhca4IIIAtT+YCX:S:M:T/?P0.97
AtpDIVAtT+YCS:T:L:M/?P0.88
PsbRIAtT+YCP:I:T:X/−P0.86
PetCIVAtT+YCT:S:R:L/+P0.63
PsbRSoT+YCT:P:I:S/+P0.64
PsbRNtT+YCP:T:I:X/?N0.78
Lhca2IIIAtT+YCX:O:W:E/+P0.98
Lhcb6IAtT+YMX:O:W:E/?P0.99
Lhcb6SoT+YCS:X:T:L/+P0.97
PetMIIAtT+YCI:T:P:M/?P0.01
PsbSSoT+YCP:G:W:E/−P0.60
Lhcb2PsT+YCN:X:T:S/−N0.93
PsaHIIIAtT+YCS:X:T:L/+M0.84
PsaHIAtT+YCS:X:T:L/+N0.93
PsaHSoT+YCC:M:X:T/−P0.74
Lhcb2IIβAtT+YCT:X:S:L/+P0.93
PetCSoT+YCL:S:T:M/+P0.16
AtpCIVAtT+YCS:T:L:M/+P0.80
Lhcb3PsT+YCI:X:P:M/−B0.70
Lhcb2IIIAtT+YCW:X:T:P/?P0.96
Lhcb1AtT+YCS:X:T:L/+N0.88
Lhcb1AtT+YCS:T:X:L/+P0.83
Lhcb1AtT+YCS:T:X:L/+P0.83
Lhcb1II1AtT+YCX:S:M:T/?P0.84
Lhcb1–50NtT+YCS:T:L:M/+N0.81
Lhcb3VAtT+YCT:I:X:S/+P0.32
PsbWSoT−YCT:I:S:L/+P0.93
PsbXcIIAtT−YCI:T:P:R/?P0.84
PsbWIIAtT−YCI:T:P:R/+P0.66
PsbOPsLsYML:R:S:M/+N0.45
PsbOIIIAtLsYCL:R:S:T/+P0.85
PsbOVAtLsYCL:S:R:T/+P0.30
PsaNAtLtYCP:W:G:E/?P0.29
PsbQSoLtYCL:R:S:T/+N0.54
PsbQIV1AtLtYCL:C:S:X/?P0.24
PsbQIVαAtLtYCL:R:S:M/?P0.18
PsbXIIIAtLtYCR:M:I:Q/−P0.45
PsbPSoLtYCL:S:T:N/+N0.39
PsbPIAtLtYCL:S:R:T/+P0.63
PsbPPsLtYCR:C:M:T/−N0.44
  • a A. thaliana and tobacco protein annotations as described in Tables II and III. Pea and spinach annotations using standard genes.

  • b At, A. thaliana; Ps, Pisum sativum; So, Spinacia oleracea; Nt, N. tabacum.

  • c Chloroplast localization. S, stroma; T+, thylakoid membrane via the signal recognition particle-dependent pathway; T−, thylakoid membrane via the spontaneous pathway; Ls, lumen via the Sec pathway; Lt, lumen via the Tat pathway.

  • d Prediction of protein targeting using PSORT. E, endoplasmic reticulum lumen; W, endoplasmic reticulum membrane; X, peroxisome; P, plasma membrane; N, nucleus; Q, mitochondria outer membrane; I, mitochondria inner membrane; R, mitochondria intermembrane space; M, mitochondria lumen; S, chloroplast stroma; T, chloroplast thylakoid membrane; L, chloroplast lumen; O, secreted; V, vacuole; C, cytosol; G, Golgi body. The correct localizations are in bold. The PSORT hydrophobic moment analysis for chloroplast proteins output is indicated by positive (+), negative (−), or unknown (?).