Table I

Filter parameters used in the SEQUEST analysis of the ms/ms spectra generated for peptides within the LMW serum proteome

Charge StateXcorrProteolytic Constraint
+1≥1.9Fully tryptic
+1≥2.1Chymotryptic and/or elastic
+1≥2.2Partially tryptic, chymotrytpic, and/or elastic
+2≥2.2Fully tryptic
+2≥2.4Partially tryptic, chymotrytpic, and/or elastic
+2≥3.0None
+3≥3.75Tryptic, chymotrytpic, and/or elastic only