Table II

GPI-APs identified by modification-specific proteomic analysis of A. thaliana plasma membranes

AGI, Arabidopsis Genome Initiative code. B, protein band number on SDS-PAGE gel. #, number of unique peptides matched for each protein/protein family. Score, score obtained in MASCOT database search (significance threshold of 54). MPSS, comparison of estimated protein levels and available expression data. Quantitative expression data for the genes in callus tissue (in ppm) have been obtained by Massive Parallel Signature Sequencing (MPSS, mpss.ucdavis.edu/java.html) where/means no data available. ESTs, expressed sequence tags. The number of published ESTs corresponding to the genes has been obtained from the Munich A. thaliana database (MATDB, mips.gsf.de/proj/thal/db/index.html). DGPI (www.expasy.org/tools), big-PI (mendel.imp.univie.ac.at/gpi/gpi_server.html), and Borner et al.: Proteins were recognized as GPI-APs after searching with these predictors and after reviewing the list of A. thaliana GPI-APs predicted by Dupree and co-workers (mips.gsf.de/proj/thal/db/index.html). A filled circle means that a protein has been recognized as a GPI-AP, and an open circle means that a protein has not been recognized as GPI-AP.

Protein identificationGPI-AP prediction
AGI codeBProtein family#ScoreMPSSESTsDGPIbig-PIBorner et al. (Ref. 18)
At1g328609β-1,3-glucanase257/2
At1g647606β-1,3-glucanase11300300
At2g2750011β-1,3-glucanase312389
At3g135604β-1,3-glucanase294/3
At4g293604β-1,3-glucanase139/1
At4g311408β-1,3-glucanase155331083
At5g0800016β-1,3-glucanase37306
At5g421009β-1,3-glucanase3634517
At5g565905β-1,3-glucanase2106135
At5g584805β-1,3-glucanase8375/3
At5g6113016β-1,3-glucanase2661402
At2g016304β-1,3-glucanase and EGF-like domain832705
At4g252404BP10-like, pectinesterase family11472211
At4g124203BP10-like/SKU518879/12
At5g514807BP10-like/SKU5419313
At3g298105COBRA162/0
At4g161202COBRA3106/4
At5g6713010Contains PLC-X domain7354405
At3g073902DoH domain51746013
At2g454702Fasciclin-like73551538
At3g609005Fasciclin-like299124
At5g557304Fasciclin-like164011
At5g441307Fasciclin-like1141704
At2g047803Fasciclin-like AGP3642827
At4g1273013Fasciclin-like AGP-23117540
At3g465505Fasciclin-like SOS5136161
At4g266901Glycerophosphodiesterase-like17698015
At5g554801Glycerophosphodiesterase-like1470709
At5g580907Glycerophosphodiesterase-like1601009
At1g747905Hedgehog-interacting protein (HIP)-like4111495
At2g138202Lipid-transfer protein-like (LTPL)271/8
At2g271309Lipid-transfer protein-like (LTPL)13660
At5g640801Lipid-transfer protein-like (LTPL)143347
At4g275201Phytocyanin-like (early nodulin-like)110412925
At5g250901Phytocyanin-like (early nodulin-like)2104140
At5g2023015Phytocyanin-like (stellacyanin-like)2411568
At5g1535015Plastocyanin-like domain13030515
At3g157209Polygalacturonase4150440
At5g100805Protease27000
At1g776301Receptor-like kinase28087
At2g171208Receptor-like kinase7273119
At1g619004Unknown263439
At4g281009Unknown292/4
At5g1925014Unknown151015