Table II

Measured m/z and calculated MH+ values of peptides found in tryptic digest of the 46-kDa protein

M* represents oxidized methionine residues; Ac-SDN...is the acetylated N-terminal of the protein; Q** represents the N-terminal Gln residues converted into pyro-Glu; and N*** represents an Asn residue converted into Asp due to deamidation

m/z measuredMH+ calculated (Da)Δm (mDa)Residues start-endPeptide sequence
601.303601.305−3204–209GNSPAR
601.322601.3311103–107ELSPR
698.357698.358−1144–149DHIGTR
708.330708.335−5 96–102GGDGKM*K
711.333711.3312294–299QGTDYK
746.387746.3834356–361HIDAYK
 749.354749.3540178–185GGSQASSR
805.378805.380−2196–203NSTPGSSR
831.462831.4575227–233LNQLESK
876.452876.461−9101–107M*KELSPR
886.401886.406−5170–177GFYAEGSR
916.478916.4780362–369TFPPTEPK
928.543928.546−3348–355DNVILLNK
946.513946.5112 62–68EELRFPR
1105.5481105.553−5339–347LDDKDPQFK
1144.4931144.499−61–10Ac-SDNGPQSNQR
1154.5791154.580−1376–385TDEAQPLPQR
1166.5571166.559−2267–276Q**YNVTQAFGR
1183.5891183.5863267–276QYNVTQAFGR
1202.6101202.613−3238–248GQQQQGQTVTK
1282.6781282.6753375–385KTDEAQPLPQR
1330.6981330.708−10238–249GQQQQGQTVTKK
1410.7741410.7713376–387KKTDEAQPLPQR
1611.6981611.6926406–421QLQNSM*SGASADSTQA
1684.8951684.8914128–143EGIVWVATEGALNTPK
1687.8981687.905−7210–226MASGGGETALALLLLDR
1703.8971703.900−3210–226M*ASGGGETALALLLLDR
1774.8381774.8362278–293GPEQTQGNFGDQDLIR
1850.8331850.8276 15–32ITFGGPTDSTDNNQNGGR
1851.8141850.8113 15–32ITFGGPTDSTDNNQN***GGR
1875.8791875.8790389–405Q**PTVTLLPAADM*DDFSR
1892.9051892.906−1389–405QPTVTLLPAADM*DDFSR
1930.9441930.9377277–293RGPEQTQGNFGDQDLIR
2005.0082005.0062388–405KQPTVTLLPAADMDDFSR
2015.0812015.0810339–355LDDKDPQFKDNVILLNK
2021.0052021.0014388–405KQPTVTLLPAADM*DDFSR
2077.0482077.0435320–338IGM*EVTPSGTWLTYHGAIK
2091.1262091.120−2150–169NPNNNAATVLQLPQGTTLPK
2151.0022151.0106 69–88GQGVPINTNSGPDDQIGYYR
2252.0622252.071−9300–319HWPQIAQFAPSASAFFGMSR
2297.0782297.092−14108–127WYFYYLGTGPEASLPYGANK
2307.1282307.11117 69–89GQGVPINTNSGPDDQIGYYRR
2324.1872324.190−3 41–61RPQGLPNNTASWFTALTQHGK
2516.3252516.339−14210–233M*ASGGGETALALLLLDRLNQLESK