Table III

Deglycosylated peptides found in PNGase F-treated tryptic digest of 139-kDa spike protein

Bold N symbols represent deglycosylated sites.

PeptideSequenceCalc. [M+H]+ with no glycosylationm/z measured after PNGaseΔmNo. of sites
T111–126SQSVIIINNSTNVVIR SQSVIIINNSTNVVIR1756.9921757.973 1758.9630.981 1.9711 2
T222–232LPLGINITNFR1257.7321258.7150.9831
T226–287YDENGTITDAV DCSQNPLAELK2453.1142454.1010.9871
T316–333FPNITNLCPF GEVFNATK2069.0172071.0011.9842
T778–796YFGGFNFSQ ILPDPLKPTK2169.1382170.1210.9831
T1056–1068NFTTAPAICHEGK1445.6851446.6720.9871
T1074–1089EGVFVFNG TSWFITQR1887.9391888.9310.9921
T1140–1163NHTSPDVDLGDI SGINASVVNIQK2493.2592495.231.9712
T1174–1187NLNESLIDLQELGK1585.8441586.8230.9791