Table IV

Glycosylated peptides found in tryptic digest of 139-kDa spike protein without treatment with PNGase F

Peptide symbols: N, first glycosylation site; N, second glycosylation site. Carbohydrate composition symbols: M, mannose; F, fucose; G, galactose; N, N-acetylglucosamine. *, m/z values could not be determined accurately (see text). **, Δm, measured - calculated mass.

PeptideSequenceCalc., [M+H]+m/zm/z measured glycopeptideResidual carbohydrate massCarbohydrate compositionCalculated carbohydrate massΔm**MS/MS glycopeptide
T111–126SQSVIIIN1756.9923201.5141444.522M3N4F1444.534−0.012
NSTNVV3404.5721647.580M3N5F1647.613−0.033
IR3525.6691768.677M3N4G2F1768.6400.037
3566.6701809.678M3N5GF1809.6660.012
3607.6821850.690M3N6F1850.693−0.003
3728.6871971.695M3N5G2F1971.719−0.024
3769.6782012.686M3N6GF2012.745−0.059
3890.7612133.769M3N5G3F2133.772−0.003
3931.7932174.801M3N6G2F2174.7980.030
4093.8182336.826M3N6G3F2336.859−0.025
4255.8442498.852M3N6G4F2498.9040.052
4296.9052539.913M3N7G3F2539.930−0.017
T222–232LPLGINI1257.7322636.2151378.483M6N21378.4760.007
TNFR2798.261540.528M7N21540.5280.000
2960.3061702.574M8N21702.581−0.007
3122.3731864.641M9N21864.6340.007
T226–287YDENGT2453.1144100.6661647.552M3N5F1647.613−0.061
ITDAVD4303.7351850.621M3N6F1850.6930.072
CSQNPL4427.1*1974.0M3N5FG21971.719
AELK4468.9*2015.8M3N6FG2012.745
4589.1*2136.0M3N5G3F2133.772
4792.2*2339.1M3N6G3F2336.859
4953.9*2500.8M3N6G4F2498.904
T778–796YFGGFN2169.1383385.591216.452M5N21216.423−0.029
FSQILPD3547.6441378.506M6N21378.476−0.030
PLKPTK3709.7381540.600M7N21540.529−0.071
3871.7021702.564M8N21702.5810.017