Detection limits and mass accuracy of ESI-MS analyses of polyacrylamide gel-eluted standard proteins
Molecular masses have been determined from the deconvolution of mass spectra in the m/z range 700–1900 and represent the average of three independent analyses.
| Proteins | Theoretical molecular mass (Da) | Experimental molecular mass (Da) | Mass accuracy (Da) | Detection limit (pmol) |
|---|---|---|---|---|
| Myoglobin | 16952 | 16952 | ±1 | 6 |
| Carbonic anhydrase | 29022 | 29022 | ±1 | 7 |
| Protein A | 44622 | 44624 | ±2 | 5 |
| Enolase | 44672 | 44672 | ±1 | 6 |