Table I

Detection limits and mass accuracy of ESI-MS analyses of polyacrylamide gel-eluted standard proteins

Molecular masses have been determined from the deconvolution of mass spectra in the m/z range 700–1900 and represent the average of three independent analyses.

ProteinsTheoretical molecular mass (Da)Experimental molecular mass (Da)Mass accuracy (Da)Detection limit (pmol)
Carbonic anhydrase2902229022±17
Protein A4462244624±25