Table I

Up-regulated proteins in p53-induced apoptosis

CategoryProtein descriptionNCBI nonredundant accession numberH:L ratioaS.D.bNumber of matched peptidesNumber of precursor-containing peptidesMethodc
Cell-cycle arrest and p53 binding14-3-3 σgi 3989532.210.1954A
Protein chaperoning and configurationHeat shock protein 70 (HSP70)gi 4623251.430.121310A
Heat shock protein 27 (HSP27)gi 198550732.680.2743A
Heat shock cognate protein 54 (HSC54)gi 115265731.430.0697A
Cyclophilin Agi 1181021.360.1176A,B
Cyclophilin Bgi 1180901.420.0765A
Plasma membrane dynamicAnnexin Igi 1139441.250.0488A
Annexin IIgi 1139501.510.22139A,B
Annexin A4gi 17033191.580.1587A
Annexin Vgi 1139601.280.081511A
Stress responseGlucose-regulated protein 78 (GRP78)gi 149169991.820.071210A
Glucose-regulated protein 94 (GRP94)gi 1193601.510.081311A
Oxygen-regulated protein (ORP150)gi 107201851.550.121212A
Protein disulfide isomerase (PDI) A3gi 25074611.400.0277A
Protein disulfide isomerase (PDI) A4gi 1195301.220.0733A
Protein disulfide isomerase (PDI) A6gi 25012051.240.1044A
Calpain L1gi 124086561.350.0577A
Antioxidant enzymesPeroxiredoxin 2gi 25071691.310.0354A
Peroxiredoxin 5gi 201417131.290.0933A
Glutathione-S-transferase P (GST P)gi 1217461.310.1055A
Glutathione-S-transferase omega 1gi 47584842.790.2143A
Glycolysis, ATP generation and transportGlyceraldehyde-3-phosphate dehydrogenase (GAPDH)gi 1206491.250.0243A,B
Pyruvate kinase M1gi 201782961.510.041211A,B
Pyruvate kinase M2gi 1256041.480.0587A,B
Lactate dehydrogenase A chain (LDH A)gi 1260471.330.12107A
Lactate dehydrogenase B chain (LDH B)gi 1260411.360.0643A
Isocitrate dehydrogenase (ICDH)gi 201415681.3021A
Mitochondrial ATP synthase α subunitgi 1145171.490.1588A
Mitochondrial ATP synthase β subunitgi 1145491.420.081814A
Adenine nucleotide translocator (ANT)gi 1134552.150.0344A
Voltage-directed anion channel 1 (VDAC-1)gi 1306831.430.031210A
OthersSialic acid synthasegi 120564731.570.0732A
Pyrroline-5-carboxylate synthase (P5CS)gi 185744581.470.1165A
Transgelin (SM 22a)gi 5860002.850.3044A
ATP-dependent DNA helicase II, 70 kDa subunit (ku70)gi 1257291.260.041010A
  • a H:L ratio, intensity ratio of heavy isotope peak versus light isotope peak.

  • b S.D., standard deviation determined from quantification ratio from several peptides.

  • c A, μLC-nanospray-MS/MS; B, MALDI-TOF MS.