Table I

List of identified proteins by MALDI-TOF MS and their annotations

Spot no.ProteinAbb.Coverage (%)Accession no.Molecular mass calculated (kDa)Predicted functionGene ontology
A1LumicanLUM18P5188538.3Extracellular MatrixGO : 0005578
A2Splicing factor 3a1SF36NP_08045175.7Splicing
A5RAB-23 (open brain)R2321P3528826.7TransportGO : 0006886
A6Hsp110h11013Q6131694.1ChaperoneGO : 0003773
A8Tumor rejection AntigenTRA35P0811392.5ChaperoneGO : 0003754
A11Lamin B1LB162P1473366.9CytoskeletonGO : 0005882
A12HrnpkHnrp24Q6057751.0SplicingGO : 0030529
A14VimentinVim82P2015253.7CytoskeletonGO : 0005882
A15Tubulin α-1Tbα59P0255150.1CytoskeletonGO : 0007018
A17ATP synthase βATPβ51P5648056.6Energetic metabolismGO : 0006754
A19Enolase 1Eno60P1718247.1GlycolysisGO : 0006096
A22β-actinβac61P0257041.7CytoskeletonGO : 0005200
A23Creatin kinase brainCki55Q0444742.7GlycolysisGO : 0004111
A24–26Transducin β1Tβ141P0490137.3Vision
Transducin β2Tβ222P5431237.3Vision
A31Peroxiredoxin 2Px271Q6117121.8RedoxGO : 0016209
A32PE-binding proteinPeb30P7029620.9Signal transduction
A33Histone h2b fH2b51P1085313.9TranscriptionGO : 0005718
A35β-synucleinβSy35Q1614314.3Synaptic transmission
A37Cytochrome c oxidaseCyc13P1278716.2Energetic metabolismGO : 0006118
A42RecoverinRec51P3405723.4VisionGO : 0007601
A4314-3-3 protein ζ14ζ53P3521527.7Vision
14-3-3 protein α14α27P3194627.7Vision
A44Phosphoglycerate mutasePgM74P1866928.8GlycolysisGO : 0006096
A47EF1α1Eα122P1012650.1TranslationGO : 0003746
EF1α2Eα221P2770650.1TranslationGO : 0003746
A49PTB-splicing factorPTB27P2324676.1SplicingGO : 0006371
A55Glutamine synthaseGlS44P1510542.1Glutamate metabolismGO : 0004356
A62LDH-ALDH56P0615136.8GlycolysisGO : 0006096
A67Cyclophilin ACyA55P1774217.9ChaperoneGO : 0006457
A68UbiquitinUbn72P022488.6Protein degradation
A69Vacuolar ATPasevATP31P5051668.2Energetic metabolismGO : 0006754
A70CalretininCaR16Q0833131.4Ca2+-bindingGO : 0005509
A71S-arrestinArr47P2044344.9VisionGO : 0007601
A73Histone H2A.3H2a67P2067114.1TranscriptionGO : 0005718
A74Chromatin assembly factorCAF11Q1311261.0TranscriptionGO : 0005634
A76P 450 11B2P4508P1553957.0Steroid hormone biosynthesisGO : 0006700
A78TransaminaseTrm23P0520247.4Glutamate metabolismGO : 0006520
Acetyl-CoA acetyltransferaseAct12P2475245.2GlycolysisGO : 0003985
A79Hypothetical cytochrom c fam.ZnF12BAB2856942.9TranscriptionGO : 0005634
CDK9CDK914NP_57093042.7Transcription
A8060S protein ribosomal60S17P4042923.6TranslationGO : 0003735
40S ribosomal protein S3A40S19P9735130.0TranslationGO : 0006412
A81snRNPSnrp14P4333113.9SplicingGO : 0006371
T-box transcription factorTbx13P7032519.8TranscriptionGO : 0003700
A96Hsp 9424P4872294.3Chaperone
A104Vesicular fusion P20P4646082.5TransportGO : 0008565
A105Actin interacting protein38O8834266.4CytoskeletonGO : 0005856
A106Ulip 234O0855362.1Cell Signalling
A109Unc-18 homolog18O0859967.7TransportGO : 0008565
A113Ulip 430O3509861.9Cell Signalling
A116TACE13Q9Z0F873.8Cell Signalling
A117Dihydrolipoamide deshydrogenase21O0874954.2Energetic metabolismGO : 0006118
A134Fructose bisphosphate aldolase63P0506439.2GlycolysisGO : 0006096
Oligo synthetase33P1192842.4Immune responseGO : 0006955
A138HnRNP A229O8856936.0SplicingGO : 0030529
A139Mitochondrial malate deshydrogenase35P0824935.6GlycolysisGO : 0006099
A140Capping β25P4775730.6CytoskeletonGO : 0005200
A146UCP 220P7040633.4Energetic metabolismGO : 0006839
A150RAN34P1708024.4TransportGO : 0008565
RGS 1821Q99PG427.6Signal transductionGO : 0007165
BAD17Q6133722.1ApoptosisGO : 0006915
A151Triose P isomerase55P1775126.6GlycolysisGO : 0006096
A155Phospholipase A2 IIF27Q9QZT418.9Signal transduction
A157Lactoylglutathione lyase35Q9CPU020.7Redox
Phosphatidylinositol transfert P22P5381031.7Signal transductionGO : 0004462
B1α crystallin A, major componentαCM86P0249019.4Chaperone
B2α crystallin A, minor componentαCm43P2462222.5Chaperone
B6β crystallin B3βC360Q9JJU924.3Chaperone
B29FusFus14P5695952.7SplicingGO : 0003723
B31Carbonic anhydraseCAII54P0092029.0GlycolysisGO : 0004089
B33GDP dissociation Inhibitor 1GDI29P5093636.6Transport
B36α crystallin B chainαCB21P2392720.1Chaperone
B39Peroxiredoxin 5Px540O0870924.9RedoxGO : 0016209
Triose P isomeraseTPI30P1775126.7GlycolysisGO : 0006096
B42β crystallin B2βC240P2677523.4Chaperone
C6Tubulin β-5 chainTbβ37P0521849.7CytoskeletonGO : 0005198
C25Voltage-dependent anion channel 1VdC41Q6093236.5ApoptosisGO : 0008308
C33ISGF-3GI3G14Q6117944.6TranscriptionGO : 0003700
C42CalreticulinCaN48P1421148.0Ca2+-bindingGO : 0005514
C43Valosin containing ProteinVCP43Q0185389.3TransportGO : 0006810
MetalloendopeptidaseMep10P7066986.4Protein degradationGO : 0006508
C45Hsp86-1 αH86α45P0790184.8ChaperoneGO : 0003773
Hsp86-1 βH86β28P1149983.3ChaperoneGO : 0003773
C46Hspa5ha555P2002972.4Chaperone
C48Hspa8ha846P0810970.9ChaperoneGO : 0003773
Hsp70.1h7024P1787970.0ChaperoneGO : 0003773
C49PDIPDI47P0910357.1Redox
C50PhosducinPho61Q9QW0828.0VisionGO : 0007601
Proliferin related proteinPrp21P0476927.9Cell signallingGO : 0005179
C5114-3-3 protein ε14ε70P4265529.2Vision
C53HMG-1HMG51P0715524.9TranscriptionGO : 0006355
C54K-rasras22P3288321.7Signal transductionGO : 0007264
C56GAPDHGdh74P1685835.8GlycolysisGO : 0006096
C58Acetyl glucosaminyl transferaseagt18P9740245.5Extracellular matrix
C63Cytoplasmic malate deshydrogenaseMdh47P1415236.5GlycolysisGO : 0006899
C65Glutamate oxaloacetate transaminase 1Got39P0520146.2Glutamate metabolismGO : 0006520
Aldolase 3CAld33P0506325.0GlycolysisGO : 0006096
C67Phosphoglycerate kinasePhK56P0941144.5GlycolysisGO : 0006096
Mitonchondrial creatin kinaseCKm38P3027547.0GlycolysisGO : 0004111
C69Glutamate deshydrogenaseGld10P2644361.3Glutamate metabolismGO : 0004353
C70ATP synthase αATPα31Q0326559.8Energetic metabolismGO : 0006754