Table II

Identification from MS/MS and LC-MS/MS analysis

SpotsAnalysisPrecursor ion (charge)Fragment ions*Identified sequenceIdentified proteinAccession no.Molecular mass calculatedPredicted functionGene ontology
A45MS/MS640.3 (2+)y10,y9,y8,y7,y2,y1GEHPGXXXAKHMG-1P0942924.9TranscriptionGO : 0006365
A50MS/MS611.3 (2+)y4,y3,y2,y1VLTKPyruvate kinaseP5248057.9GlycolysisGO : 0006096
586.0 (2+)b1,b2,b3,b4,b5,b6LDIDSA
C13MS/MS564.4 (1+)y3,y2,y31FELRATF-2P1695142.0TranscriptionGO : 0006365
A77LCMS/MS513.4 (2+)y9,y8,y7,y6,y5,y4,y2,y1IGGIGTVPVGRTranslationGO : 0003746
488.3 (2+)y7,y6,y5,y4,y3,y2,y1LPLQDVYKEF-1αP3482349.3
A83LCMS/MS542.9 (2+)y10,y9,y8,y7,y6,y5,y4,y3,y2,y1GSPLVVISQGKUlip2O0855362.1Cell signalling
B46LCMS/MS898.0 (2+)y9,y6,y4,y1/ b7,b8,b9MLSDGRTIITFPNGTRTcp-10S2641349.5Immune response
B47LCMS/MS713.9 (2+)y10,y9,y8,y7,y6,y4,y2,/b2,b6VTVYELENFQGKβ crystallin B3Q9JJU924.3Chaperone
  • * ion fragments are labelled according to the nomenclature of Roepstorff (31). y and b fragments correspond to rupture of the peptidic bond with charge carried on the C and N terminus, respectively. The accompanying number corresponds to the position of the amino acid in the sequence.