Table I

Selected proteins identified on WGA beads after incubation with apical RPE

ProteinsAccession no.aPeptides matchedFunctionbExperimentc
Actin, cytoplasmic 1 (β-actin) P60710 11CYT++
Actin, γ Q9QZ83 19CYT+++
α enolase P17182 5E+++
Annexin A2 P07356 2MT+
Annexin A5 P48036 3MT+
β enolase P13929 3E++
Basigin P18572 6MT+++
Carbonic anhydrase XIV Q9WVT6 2E+
Chloride intracellular channel 6 Q96NY7 2MT+++
Cellular retinaldehyde-binding protein (CRALBP) Q9Z275 6RM+++
Cytokeratin 15 I49595 5CYT+++
Decorin P28654 4ECM++
Dermcidin P81605 3ECM+
EBP50 Q9JJ19 1dCYT+
Ezrin P26040 4CYT++
Fibromodulin P50608 4ECM++
Fructose-bisphosphate aldolase A P05064 4E+++
Glut-1 P17809 3MT+++
GST P P04906 2E+
Glyceraldehyde 3-phosphate dehydrogenase P16858 8E+++
Glycogen phosphorylase Q9WUB3 5E++
Interphotoreceptor retinoid-binding protein (IRBP) P49194 5RM+++
l-lactate dehydrogenase A chain (LDH) P06151 3+
Lumican P51885 9ECM+++
Malate dehydrogenase P14152 2++
Membrane-associated adenylate kinase Q9R0Y4 2MT+
Moesin P26041 3CYT++
Monocarboxylate transporter 1 AAC13720 3MT+++
Monoglyceride lipase O35678 2E++
Na,K-transporting ATPase α1 chain P06685 6MT+++
Neuroglycan C Q9QY32 1cECM+
Neuronal membrane glycoprotein M6-a P51674 1MT+
Peroxiredoxin 1 Q63716 1cE+
Peroxiredoxin 2 Q9CWJ4 2E+
Phosphoglycerate kinase P09411 2E++
Profilin I P10924 2CYT+
Pyruvate kinase, M1 isozyme P14618 4E+
Pyruvate kinase, M2 isozyme P52480 2E+
Retinol-binding protein, cellular (CRBP) Q00915 5RM++
Retinol dehydrogenase, 11-cis (RDH5) Q27979 3RM+++
Sodium/potassium-transporting ATPase α-1 P06685 6MT+++
Spectrin β chain Q00963 CYT++
Tubulin α-2 chain P05213 6CYT++
Undulin 1 A40970 7ECM++
Vitronectin receptor α subunit (integrin αv) P43406 3MT++
Totale8697100
  • a Swiss-Prot database and NCBI (in italics) accession numbers are shown; for links use the EXPASY server at us.expasy.org/sprot/ and www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=protein&cmd=search&term=.

  • b Functions: CYT, cytoskeleton and cytoskeleton-associated; E, enzyme; ECM, extracellular matrix; MT, plasma membrane and transporter; RM, retinoid metabolizing.

  • c WGA-coated beads were incubated on the apical RPE surface in mouse eyecups, recovered, washed, and bound proteins identified as described in the text. Selected results from three experiments are shown from 16 mouse eyes each.

  • d The identified peptide sequences: EBP50, AVDPDSPAEASGLR; peroxiredoxin 1, GLFIIDDKGILR; neuroglycan C, ETGSAIEAEELVR.

  • e Total of proteins identified by LC MS/MS and bioinformatics in each preparation.