Table I

Known metal-binding salivary proteins

Masses were derived from cleavage of precursors, based on cleavage sites information presented in the Swiss-Prot and TrEMBL data base on the EXPASY website.

Note: These studies were repeated approximately 25-times over a 6-month period of time. All studies produced results comparable to those shown in this table. Mass variance (calculated − observed) was generally less than 0.1%, which is consistent with day-to-day variations associated with MALDI-TOF MS analysis using external calibration.

ProteinSwiss-Prot accession no.ResiduesMassMass + 1PMass + 2P
PRP C HUMANcalcP028101–16617,017
PRP CobsNot observed
PRP1/2calca17–16615,372.315,452.315,532.3
PRP1/2obs15,372.715,452.415,532.6
PRP3/4calcb17–12211,019.511,099.511,179.5
PRP3/4obs11,016.011,095.811,176.5
Peptide P–Ccalc123–1664370.8
Peptide P–CobsNot observed
STAT_HUMANcalcP28081–627304.57384.57464.5
STAT_HUMANobsNot observed
Statherincalc20–625219.75299.75379.7
Statherinobs5307.85388.1
HIS1_HUMANcalcP155151–576962.9
HIS1_HUMANobsNot observed
Histatin 1calc20–574848.24928.2
Histatin 1obs4856.24936.0
HIS3_HUMANcalcP155161–516149.1
HIS3_HUMANobsNot observed
Histatin 3calc20–514062.4
Histatin 3obs4066.1
Histatin 5calc20–433036.3
Histatin 5obs3036.1
Histatin 6calc20–443192.5
Histatin 6obs3192.9
  • a PRP1 represents three positional isomers, namely PRP-1, PRP-2, and PIF-s, using the nomenclature of Hay et al. (22).

  • b PRP3 represents three positional isomers, namely PRP-3, PRP-4, and PIF-f, using the nomenclature of Hay et al. (22).