Table II

Methods of detection of HER-2/neu status in breast cancer

MethodTargetFDA-approvedSlide-based
IHCProteinYesaYes
FISHGeneYesaYes
CISHGeneNoYes
Southern blotGeneNoNo
RT-PCRmRNANoNo
Microarray TPbmRNANoNo
Tumor ELISAProteinNoNo
Serum ELISAProteinYescNo
  • a For prognosis and prediction of response and eligibility to receive trastuzumab therapy.

  • b TP, transcriptional profiling.

  • c For monitoring response of breast cancer to treatment.