Table I

List of additional peptides from “SemiTrypsin” and “No Enzyme” specificity searches

The top 50 protein list from Mascot is listed along with charge states, Mascot scores, and peptide sequences for the additional peptides. Peptides found to be fully tryptic are italicized.

Protein hit no.Accession and descriptionSemiTrypsin sequenceChargeScoreNoEnzyme sequenceChargeScore
1Q8C196, carbamoyl-phosphate synthase(G)ISTGNIITGLAAGAK(S)253
(W)PANLDLKK(E)248
(D)PNKQNLIAEVSTK(D)273
2P16460, argininosuccinate synthase(D)PAKAPNSPDVLEIEFKK(E)331
6P26443, glutamate dehydrogenase(Y)SEAAADREDDPNFFK(M)328
11P54869, 3-hydroxy-3-methyl-glutaryl-coenzyme A synthase (R)GLKLEETYTNKDVDKALLK(A) 328
17Q9ET01, glycogen phosphorylase (R)IKKDPKK(F) 234
19Q91ZV9, carboxylesterase 3(G)YPSSPPVVNTVK(G)237
20P07724, serum albumin(D)FAEITKL(A) (D)LGEQHFKG(L)230
44P01027, complement C3 (HSE-MSF)(N)ALKLQK(G)242
48P08113, endoplasmin(A)DDEVDVDGTVEEDLGKSR(E)327 (K)EGVKFDESEKTKESR(E) 328
50Q91YI0, argininosuccinate lyase(D)LILYGTKEF(S)233