Table I

Twenty-five most highly upregulated microsomal proteins between low (PC3M) and high (PC3M-LN4) metastatic prostate cancer cells, as determined by SILAC

Protein nameGenBank acc. no.aSILAC ratiob
Polyadenylate-binding protein 1318354441.78 ± 11.68 (3)
Talin423527516.54 (1)
Chaperonin (HSP60)30689013.85 ± 1.56 (4)
Laminin-binding protein3423413.16 ± 1.82 (2)
Eukaryotic translation initiation factor 3, subunit 10 θ, 150/170 kDa45035099.71 ± 2.69 (4)
Vimentin3402199.55 (1)
Developmentally regulated GTP-binding protein 147587969.48 (1)
Annexin I45021018.28 ± 1.31 (5)
Malate dehydrogenase, mitochondrial precursor66480677.93 ± 2.17 (2)
B-cell receptor-associated protein16735147.23 (1)
Proliferation-associated 2G4, 38 kDa54538427.22 ± 1.21 (7)
Translation initiation factor eIF-2 β chain879416.58 ± 1.37 (4)
Annexin XI45573176.56 ± 0.65 (3)
Citrate synthase precursor47580766.27 ± 1.24 (2)
ATP synthase, H+-transporting, mitochondrial F1 complex, β polypeptide321893945.92 (1)
Voltage-dependent anion-selective channel protein 1107202165.75 ± 0.83 (2)
Unnamed protein product227607625.71 ± 0.59 (5)
Peroxiredoxin 3 isoform a precursor58029745.69 (1)
Phenylalanine-tRNA synthetase-like protein47583405.67 ± 1.45 (2)
Phenylalanyl-tRNA synthetase β chain126438185.43 ± 0.69 (4)
NSAP1 protein50315125.43 ± 0.11 (2)
Lysyl-tRNA synthetase110959095.20 ± 0.66 (2)
Annexin VII isoform 145021115.13 (1)
DNA-binding protein1819144.94 ± 1.04 (2)
Translation elongation factor EF-Tu-like protein P43 precursor, mitochondrial74433844.81 (1)
  • a GenBank accession number.

  • b SILAC ratio refers to fold increase in protein expression, as described in “Experimental Procedures,” with number of lysine-containing peptides quantified from each protein shown in parentheses.