Table II

Protein identifications for spots whose summer levels are greater than entrance

Listed are the group identification as assigned by Melanie 4 during analysis (ID); 2-tailed t-test p value, all are p < 0.05; *, p < 0.01; **, p < 0.001; the average fold change in mean % volume SA/Ent (Fold change); number of gels in which a spot is clearly defined (n, SA) (n, Ent); protein identification(s); abbreviation of the protein name (Abbrev.); the molecular weight of the human homolog (MW); the number of peptides recovered from the database (No. of pept.) (Note: for the method for peptide counting, see “Experimental Procedures”); a representative NCBI gi accession number for one of the protein matches (Accession); and the species for the accession number given (Species). If multiple IDs were obtained for a spot, the names were listed in separate rows. For these, the group ID, p value, fold change, and n columns were left blank as they are the same as that of the preceding row. A complete list of peptides and their mass spectral parameters is available in a supplemental table. For ID 349, the MW given is for the rat homolog rather than human. In No. of pept. column, * indicates the inclusion of ground squirrel peptides in the count.

IDpFold changen, SAn, EntProtein identification(s)Abbrev.MWNo. of pept.AccessionSpecies
1521.685610-THF synthaseC1TC1014285 13699868 Human
Eukaryotic translation elongation factor 2EF2953382 4503483 Human
153**1.4789C-1 tetrahydrofolate synthase (10-THF synthase)C1TC1014289 13699868 Human
1541.389910-THF synthaseC1TC10142810 13699868 Human
Eukaryotic translation elongation factor 2EF2953382 4503483 Human
1653.239410-THF dehydrogenase10-FTHFDH986998 11968144 Rat
169*5.229710-THF dehydrogenase10-FTHFDH9869918 24418394 Mouse
1737.287310-THF dehydrogenase10-FTHFDH9869912 6225390 Human
2031.8488Dimethylglycine dehydrogenaseM2GD968117 24797151 Human
Histone H4H4113673 4504301 Human
271*1.3999Tumor-necrosis factor activating protein (hsp75)TRAP1800104 7706485 Human
286*1.7198Annexin 6ANXA6757422 25071858 Mouse
2931.6599Glucose-regulated protein 75GRP75736802 24234688 Human
3061.2099Succinate dehydrogenase flavoprotein subunitDHSA726914 20908717 Mouse
3491.8388Carboxylesterase 3EST3621471 31542380 Rat
368**1.4399Heat shock protein 60 (chaperonin)HSP606105512 14603309 Human
370**1.3899Heat shock protein 60 (chaperonin)HSP60610559 129378 Hamster
4671.2499Glyceraldehyde 3-phosphate dehydrogenaseGAPDH359225 13702286 Guinea pig
Psoriasin (Ca-binding protein)PSOR1104173 12053626 Human
4961.5497Dihydrolipoamide branched chain transacylaseDBT534872 4503265 Human
502*1.7799Aldehyde dehydrogenase 1A1ALDH1A1547308 21654729 Rabbit
5521.4299Phenylalanine hydroxylasePH4H518617 4557819 Human
559*1.4299Phenylalanine hydroxylasePH4H518613 20070771 Human
6231.3599Succinate-CoA ligase GDP forming, beta subunitSUCLG2412197 38086922 Mouse
6571.6599Acyl-CoA dehydrogenase, C2 to C3 short chain precursorACADS442972 4557233 Human
660**1.8389Acyl-CoA dehydrogenase short/branched chain precursorACADSB474852 4501859 Human
6622.3254Acyl-CoA dehydrogenase short/branched chain precursorACADSB474855 4501859 Human
Isovaleryl CoA dehydrogenaseIVD463193 9789985 Mouse
7571.3099Aldo keto reductase family 1 member C4AKR1C4370952* 15216337 Monkey
7711.4987Aldo keto reductase family 1 member C4AKR1C4367886* 4503285 Human
Malate dehydrogenase 1, NADMDHC365113* 37589957 Mouse
878**3.3787Nit protein 2NIT2305807* 9910460 Human
1006*1.2899Peroxiredoxin 3PRDX3256924 11968132 Rat
11251.5899ThioredoxinTXN116063 20140745 Monkey