Table IV

Putative functional groupings of proteins that change in steady-state level with the hibernation season

The functional pathways are in bold. Proteins demonstrating a winter increase are listed in the left column, a winter decrease in the right column. Following each protein name is the fold change for that spot and the statistical significance taken from Tables I and II. All are p < 0.05; *, p < 0.01; **, p < 0.001. If no fold change is given, the corresponding spot contained more than one identified protein. If more than one fold change is given, more than one spot gave the same protein ID.

Entrance > summer activeSummer active > entrance
TCA cycle
    Malate dehydrogenase 1, 1.24**Succinate-CoA ligase GDP forming, beta subunit, 1.35
    Succinyl coA ligase ADP forming, 1.35*Succinate dehydrogenase flavoprotein subunit, 1.20
Amino acid and protein synthesis
    Eukaryotic translation initiation factor 4A1, 1.34
    Small ribosomal protein P40, 1.12
    Transaldolase 1, 1.58*
    UNR interacting protein, 1.36
Detoxification
    Aldehyde dehydrogenase family 1 member B1, 1.44Aldehyde dehydrogenase 1A1, 1.77*
    Aldehyde dehydrogenase 2, 1.37
    Aldehyde dehydrogenase family 7 member A1, 1.53
    Glutathione S-transferase mu 2, 1.30**
    Phenolsulfotransferase, 2.22*
Gluconeogenesis
    Malate dehydrogenase 1, 1.24**
    Phosphoenolpyruvate carboxykinase 2, 1.29
    Pyruvate carboxylase, 2.41, 1.88
Nucleotide synthesis
    Transaldolase 1, 1.58*10-THF dehydrogenase, 3.23, 5.22*, 7.28
    UMP CMP kinase, 1.53**C-1 tetrahydrofolate synthase (10-THF synthase), 1.47**
Redox balance
    Glutathione S-transferase mu 2, 1.30**Glucose-regulated protein 75, 1.65
    Peroxiredoxin 2, 1.44Peroxiredoxin 3, 1.28*
    Peroxiredoxin 4Thioredoxin, 1.58
    Protein disulfide isomerase, 1.33**
    Transaldolase 1, 1.58*
Electron transport chain
    Electron transfer flavoprotein-ubiquinone oxidoreductase, 1.54
    NADH coenzyme Q reductase, 1.52*
Protein folding and transport
    BiP, 1.35*Glucose-regulated protein 75, 1.65
    Chaperonin theta subunit TCP-1 containing, 1.35Heat shock protein 60 (chaperonin), 1.43**, 1.38**
    Sec 13-related protein, 1.31*Tumor-necrosis factor activating protein (hsp75), 1.39*
Lipid mobility and metabolism
    Acyl coA dehydrogenase long chain specific, 1.35*Acyl-CoA dehydrogenase, C2 to C3 short chain precursor, 1.65
    Apo A1Acyl-CoA dehydrogenase short/branched chain precursor, 1.83**
    Carboxylesterase 2, 2.60*, 1.53Aldo keto reductase family 1 member C4, 1.30
    Fatty acid-binding protein liver-specific, 3.65**, 2.82**Carboxylesterase 3, 1.83
    Hydroxy methylglutaryl Coenzyme A synthase 2, 1.96, 4.92*Dihydrolipoamide branched chain transacylase, 1.54
Ketone body formation
    Hydroxy methylglutaryl Coenzyme A synthase 2, 1.96, 4.92*
Amino acid and protein degradation
    Cathepsin B, 1.54Phenylalanine hydroxylase, 1.42, 1.42*
    26S proteasome non-ATPase regulatory subunit 9, 1.49**
    Proteasome 26S ATPase 3, 1.39*
    Proteasome 26S ATPase 4 isoform 1, 1.31*
    Saccharopine dehydrogenase, 2.38*
Anticoagulation
    Annexin 4, 1.41*
    Annexin 5, 1.58**
Cell and organellar architecture
    Alpha tubulin, 1.26, 1.60*Annexin 6, 1.71*
    Annexin 4, 1.41*
    Beta actin, 1.52*, 1.17
    Mitofilin, 1.51
    Valosin-containing protein (ER ATPase), 1.34
Miscellaneous
    Hypothetical protein Ab2–225, 1.26Nit protein 2, 3.37**
    Interleukin 25, 1.60
    Rho GDP dissociation inhibitor
    Selenium binding protein 1, 1.79*, 1.49