Table I

Expression and purification of 12 POET positive (1–12) and negative (13–24) ORFs

Lane numbers refer to Fig. 3. ND, not determined.

Lane no.Protein annotationSwiss-Prot accession no.Protein molecular weightaYield
Small scaleLarge scale
1TAB1-like protein TAP-1 Q93375 47,73537ND
2Protein with tau-like repeats, isoform a O02592 53,6825ND
3Machado-Joseph disease-like protein O17850 40,13010ND
4Hypothetical protein C17G10.2 Q09974 53,31698100
5Skp1p homolog (SKR-12) Q22871 23,19234ND
6Hypothetical protein F09G2.9 O17406 48,5537747
7Bag1 homolog protein 1 O44739 28,27632ND
8Hypothetical protein F53F4.3 Q20728 29,707144364
9Hypothetical protein D2096.8 Q19007 39,931130374
1014-3-3-like protein 1 P41932 32,4285960
11Troponin C, isoform 2 Q09665 22,493163ND
12Troponin T Q27371 51,30780125
13Hypothetical protein F46A9.5 Q93647 2429876ND
14Mitochondrial import receptor subunit TOM20 homolog Q19766 24,69220ND
15Probable mediator complex subunit soh-1 P91869 23,7449ND
16Eukaryotic peptide chain release factor subunit 1 O16520 53,4840ND
17CCR4-NOT transcription complex subunit 7 Q17345 38,1040ND
18Probable coatomer ζ subunit O17901 25,0230ND
19Glutathione S-transferase 3 O16116 28,00131ND
20Spermatocyte protein spe-27 (precursor) P54218 19,4490ND
21CCR4-NOT transcription complex subunit 7 Q17345 38,1040ND
22Myoblast determination protein 1 homolog P22980 40,7150ND
23G2/mitotic-specific cyclin A1 P34638 59,7760ND
24Hypothetical protein B0035.11 Q17431 52,3290ND
  • a Predicted molecular weight of the protein expressed in pDest527.