Table II

List of regulatory proteins identified by multiple peptides

Tryptic peptides from the Jurkat T cell extracts corresponding to the45–51-kDa region were analyzed both on LTQ (top 5 protocol) and LCQDeca (top 2 protocol).

Protein IDaData base annotation and functional information
GP:U94174_1Mammary tumor-associated protein INT6 gene
GPN:BC000404_1Thyroid hormone receptor interactor 13
GPN:BC009597_1Basic leucine zipper and W2 domains 2
GPN:BC004302_1COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis)
GPN:BC021139_1Nuclear distribution gene C homolog (Aspergillus nidulans)b
GPN:BC053350_1Solute carrier family 9 (sodium/hydrogen exchanger)
GPN:BC056886_1LUC7-like 2 (S. cerevisiae) (is localized to spliceosome complex in yeast)b
PIR2:A54857Transcription factor NF-AT 45-kDa chain
PIR2:JC4525Nucleic acid-binding protein E5.1
PIR2:S68209SDS22 protein homolog (a type 1 serine/threonine phosphatase)
SW:2ABA_HUMANSerine/threonine protein phosphatase 2A, 55-kDa regulatory subunit B
SW:A1M1_HUMANAdaptor-related protein complex 1, clathrin assembly protein
SW:ATXX_HUMANAtaxin-10 (involved in neurodegenerative diseases)
SW:CGL1_HUMANCytosolic nonspecific dipeptidaseb
SW:CO1A_HUMANCoronin-like protein p57 (promotes barbed end assembly of actin filaments)
SW:CSK_HUMANTyrosine-protein kinase CSK (c-Src kinase)
SW:CSN2_HUMANCOP9 signalosome complex subunit 2
SW:DCT2_HUMANDynactin complex 50-kDa subunit
SW:DD17_HUMANProbable RNA-dependent helicase p72
SW:DEK_HUMANDek protein (induces alterations in superhelical density of DNA in chromatin)
SW:ENPL_HUMANEndoplasmin precursor (gp96 homolog)
SW:FKB4_HUMANFK506-binding protein 4 (peptidyl-prolyl cis-trans isomerase)
SW:GD1A_HUMANRab GDP dissociation inhibitor αb
SW:GD1B_HUMANRab GDP dissociation inhibitor βb
SW:GLYC_HUMANSerine hydroxymethyltransferase
SW:GLYM_HUMANSerine hydroxymethyltransferase, mitochondrial precursorb
SW:HAT1_HUMANHistone acetyltransferase type B catalytic subunit
SW:HE47_HUMANProbable ATP-dependent RNA helicase p47b
SW:IEFS_HUMANStress-induced phosphoprotein 1
SW:ILK1_HUMANIntegrin-linked protein kinase 1
SW:IMA2_HUMANImportin α-2 subunit
SW:KAP0_HUMANAMP-dependent protein kinase type I-α regulatory chain
SW:LPAX_HUMANLeupaxin (most homologous to paxillin, which is found in focal adhesions)
SW:MPK1_HUMANDual specificity mitogen-activated protein kinase kinase
SW:OST4_HUMANDolichyl-diphosphooligosaccharide-protein glycosyltransferase
SW:P2G4_HUMANProliferation-associated protein 2G4b
SW:PDA6_HUMANProtein-disulfide isomerase A6 precursorb
SW:PFTA_HUMANProtein farnesyltransferase α subunit
SW:PTD4_HUMANPutative GTP-binding proteinb
SW:RBB7_HUMANHistone acetyltransferase type B subunit 2 (retinoblastoma-binding protein 7)
SW:SEP6_HUMANSeptin 6 (involved in cytokinesis)b
SW:ST24_HUMANSerine/threonine protein kinase 24 (mammalian STE20-like protein kinase 3)
SW:VAT1_HUMANSynaptic vesicle membrane protein VAT-1 homolog
SW:WD12_HUMANWD repeat protein 12
SW:YB1_HUMANCCAAT-binding transcription factor I subunit Ab
SWN:SMY3_HUMANZinc finger MYND domain-containing protein 1
  • b Acession numbers in NCBI, Swiss-Prot, or PIR database.

  • b These proteins were also identified on the 3-D ion trap (LCQ Deca) at least by a single peptide.