Table I

List of identified differentially regulated phosphopeptides

The presented ratios and standard deviations are calculated by averaging intensity-weighted SILAC ratios from consecutive FT-MS scans during the elution of the individual peptide pairs. H/L ratio, heavy to light isotope ratio.

NamePhosphopeptide sequenceH/L ratioS.D.Reciprocal ratio
NRP1AQEILpSPFPQSK0.070.0114.1
SEC16TpSPSPTGPNPNNSPSPSSPISR0.070.0114
AFR1pSNSTSNLFALSSTFSK0.070.0314.2
FUS3IIDESAADNSEPTGQQSGMTEpYVATR0.090.0110.7
FAR1TGQLSALQpSPVNITSSNK0.120.058.1
FUS3IIDESAADNSEPTGQQSGMpTEYVATR0.120.018.6
MKT1SNLSpSPSSASSSASPATTVTK0.130.017.8
NOP4VLSIpTPTLVR0.130.027.7
INP52LLpSPTKEISIVSVSPR0.140.026.9
DIG2NLQQLGMLLVpSPGLDEDRLSEK0.150.036.6
PUF4QTFHALpSPTDLINAANNVTLSK0.160.066.4
SEC10LTNVTQVVpSPLGDKLETAIK0.160.016.2
SST2NLpSNENCSFK0.160.046.1
IPP1GIDLTNVTLPDpTPTYSK0.170.035.9
RPC53EIQEALSEKPTREPpTPSVK0.180.075.5
SST2NLpSNENCpSFKK0.180.025.4
YDR261C-DAHNVSTSNNpSPSTDNDSISK0.180.015.4
CHD1GPAALINNTRLSPNSPpTPPLK0.190.035.2
PIK1SSpTPTSPIDLIDPIK0.210.044.8
STE2NQFYQLPpTPTSSK0.210.014.7
DCS1LNLIWPApTPIHIK0.220.014.6
PIK1SSTPTpSPIDLIDPIK0.220.044.6
RRP5GGASALpTPLELK0.220.024.6
STE12LVpSPSDPTSYMK0.220.074.6
YDR261C-DNVLpSK0.220.074.6
TAF4NVpSPTTNLR0.240.084.2
FUN30VASSSPLKPTpSPTVPDASVASLR0.240.034.1
SAS4NNAASIpSPTLSEK0.250.124
YDR261C-DpSPSIDASPPENNSSHNIVPIK0.250.034
FUN30VASSpSPLKPTSPTVPDASVASLR0.250.034.0
DIG1TSQPQQQpSPSLLQGEIR0.260.033.8
VPS27LTLSNpSPTAMFDSK0.260.043.9
STE20TFNTTTGLPGpSPQVSTPPANSFNK0.270.083.8
PSK1GDYGNNIpSPERPSFR0.280.043.6
YDR034C-CMESQQLSQNpSPNLHGSAYASVTSK0.280.113.5
MRH1APVApSPRPAATPNLSK0.290.053.4
SSE2/SSE1GAAFICAIHpSPTLR0.290.033.4
SST2APNGpSTIDLDFTLR0.290.043.4
TFP1GIDpTPALDR0.290.13.4
YDR034C-CVNNDHINESTVSSQYLpSDDNELSLRPATERI0.290.083.4
MRH1APVApSPRPAApTPNLSKDK0.30.043.4
LSP1ALLELLDDSPVpTPGEARPAYDGYEASR0.300.043.4
TFC7TAGLLpSPTEENETTNAGQSK0.310.093.2
WHI5NGFGTPSPPpSPPGITK0.320.033.1
YBR025CVIVPpSPR0.320.043.1
YDR261C-DTVPQIpSDQETEKR0.340.043
CDC19NCpTPKPTSTTETVAASAVAAVFEQK0.350.072.9
SEC16TNSAISQpSPVNYAFPNPYK0.360.032.8
RPL13AAPEAEQVLpSAAATFPIAQPATDVEAR0.380.092.6
SOD1FEQASESEPTTVSYEIAGNpSPNAER0.380.082.6
STB4LIpSFQMGR0.380.062.6
SHE3TNVTHNNDPSTpSPTISVPPGVTR0.390.092.6
SIS1SASSpSPTYPEEETVQVNLPVSLEDLFVGKK0.390.112.6
SSB2VTPSFVAFpTPQER0.390.012.6
YDR098C-BMESQQLSNYPHIpSHGSACASVTSK0.390.042.5
NIS1SNTpSPSVLYHPK0.40.112.5
PPQ1AGSFGAPSpSPTSGIPNPK0.40.052.5
YOR192C-AMESQQLSQNpSPTFHGSAYASVTSK0.40.092.5
DPS1AYLAQpSPQFNK0.410.042.4
NUP159SLpSPTSEKIPIAGQEQEEK0.410.12.4
VPS27ANSpSPTTNIDHLK0.410.042.4
YDR261C-DSPpSIDASPPENNSSHNIVPIK0.410.032.4
AHP1pSDLVNKK0.410.002.5
MDS3ISApSSLPIPIENFAK0.420.022.4
SMY2APIGTVVpSPYSK0.420.012.4
VIP1NNLPSAPSEMpSPLFLNK0.430.022.4
WSC2FSATpSLPDMMEER0.430.142.3
GFD1IpSPVSESLAINPFSQK0.430.052.3
HXT3MNSTPDLISPQK0.430.002.3
FAS2VVEIGPpSPTLAGMAQR0.440.062.3
CDC15SAIPSSSSLPLSSpSPTR0.450.12.2
CYR1EATpTPTIETPISCKPSLFR0.450.062.2
HXT1MNSpTPDLISPQK0.450.112.2
CUE2GIHSIGGIpSKVR0.460.052.2
PFK26RYpSVIPTAPPSAR0.460.062.2
RPG1TAGGSpSPATPATPATPATPTPSSGPK0.460.012.2
STP3SIpSPILK0.460.032.2
YOR220WGGSSLpSPDKSSLEpSPTMLK0.460.012.2
ZRG8TPIIGNENLTSTTpSPSNLEPAIR0.460.112.2
ZEO1LEETKEpSLQNK0.470.12.1
RLR1VIPPApSPSQASILTEEVIR0.480.032.1
IPP1AASDAIPPApSPKADAPIDK0.480.082.1
LSB3FTAPTpSPSTSSPK0.480.022.1
CRP1pSPALPQADDPIVETK0.490.112.1
FAR11SVpSLEDLKR0.490.12
MSN4RGpSTIpSPTTTINNSNPNFK0.490.042
SEC16ESIIpSTGSEFLPPPK0.490.122.1
ADR1FSpTPELVPLDLK0.500.052.0
GRS1YDIGNPVTGETLEpSPR0.500.052.0
YRA1SANLDKpSLDEIIGSNK8.82.650.1
DBF2LVGFpTFR6.221.950.2
RFA2GYGpSQVAQQFEIGGYVK5.760.910.2
GIN4NFpSLQTRPVSR5.552.070.2
CDC28VGEGTpYGVVYK5.031.240.2
ESC1NLpSDLENYSQR4.820.880.2
SWI4ILENpSPILYR4.790.280.2
SWI5FVISETPpSPVLK4.720.740.2
BOI2SApSEAIKGETLK4.400.710.2
COG3TKpSSSILTLAR4.020.010.2
SHS1FLNpSPDLPER3.950.250.3
SHS1NIpSETVPYVLR3.810.170.3
MCM3LQLGLRVpSPR3.730.870.3
BUD4LSSpSPLKFTLK3.630.620.3
NUP60SNVVVAETpSPEKK3.560.520.3
BEM3ALGFpSPASK3.530.400.3
MAD3QLpTPILEMR3.490.930.3
YBR255WIATpTPEAQISR3.440.530.3
GIN4LSTIVNQSpSPTPASR3.410.010.3
ORC6NQLFGpTPTK3.280.850.3
YOL070CLLSpSPLRQEK3.220.370.3
STB1SQPQMpSPEKEQELASK3.210.010.3
SHO1NTpTPYQNNVYNDAIR3.110.010.3
MSC3RLpSTSSAAPPTSR2.941.440.3
RPL12BIGPLGLpSPK2.840.240.4
BFR1KVVADDLVLVpTPK2.790.190.4
ELC1pSQDFVTLVSKDDKEYEISR2.780.010.4
MMR1NAGSFQNLLNpSPTK2.590.550.4
YMR031CKIpSGYGNDLDAQK2.570.730.4
SPA2NYWDVNDpSPIIKVDK2.480.230.4
TSL1pSATRpSPSAFNR2.460.480.4
CDC11pSGIIDASSALRK2.40.360.4
ACE2GLpSGTAIFGFLGHNK2.370.360.4
TSL1IApSPIQQQQQDPTTNLLK2.350.220.4
HBT1pSISGGTFGFR2.340.050.4
GGA1VSLSpSPKSPQENDTVVDILGDAHSK2.280.550.4
RNR1EApSPAPTGSHSLTK2.270.280.4
BUD3LGDDYpSDKETAK2.191.640.5
NET1SFLPPPTQPQpSPPIR2.180.160.5
ESC1SNIFSpSPIR2.150.280.5
CDC3QLELSINSApSPNVNHSPVPTK2.090.320.5
GFA1EVGApSMTR2.060.080.5