Table IV

Protein quantitation of the anti-pY fraction from HN5 cells by ICAT labeling and immunoblot approaches, in which the EGF receptor kinase was either inhibited with erlotinib or stimulated with EGF

Data are presented as fold stimulation. Standard errors are in parentheses. NC denotes no observable change by immunoblot.

Accession IDProtein nameErlotinib (Immunoblot) 120 minErlotinib (ICAT) 120 minErlotinib + EGF (ICAT) 60 minEGF
1 min3 min15 min
sp‖P29312 14-3-3 protein ζ (protein kinase C inhibitor protein-1)NC1.1 (0.0)1.3 (0.1)1.3 (0.1)
sp‖P05141 Adenine nucleotide translocator 2 (solute carrier family 25)1.1 (0.1)
sp‖Q14161 ARF GTPase-activating protein GIT10.9 (0.1)0.6 (0.0)0.6 (0.0)
sp‖O60716 Catenin Δ-1 (p120 catenin) (p120(ctn)) (cadherin-associated Src)1.3 (0.1)2.32.0 (0.2)
sp‖Q13191 CBL-B (SH3-binding protein CBL-B)0.31.6 (0.1)2.4 (0.1)
gi‖4758046 Cell cycle progression 2 protein1.11.1 (0.0)
sp‖P56945 CRK-associated substrate (p130Cas)0.90.8 (0.1)0.5 (0.0)0.7 (0.0)
gi‖30410805 CUB domain-containing protein 1 isoform 1 (SIMA135/CDCP1)1.1 (0.1)1.1 (0.0)0.9 (0.1)0.9 (0.1)
sp‖P04720 Elongation factor 1-α 1 (EF-1-α-1)1.21.21.5 (0.1)
sp‖P29317 Ephrin type-A receptor 2 precursor0.8
sp‖P98172 Ephrin-B1 precursor (EPH-related receptor tyrosine kinase ligand 2)1.0
sp‖Q05397 Focal adhesion kinase 1 (FADK 1) (pp125FAK; PTK2)0.40.5 (0.0)
sp‖P09382 Galectin-1 (β-galactoside-binding lectin L-14-I)1.2 (0.3)1.0 (0.1)1.5 (0.1)1.4 (0.1)
sp‖O00410 Importin β-3 subunit (Karyopherin β-3 subunit) (Ran-binding)1.0 (0.1)
sp‖Q14192 LIM-protein 3 (SLIM 3) (LIM-domain protein DRAL)1.0 (0.1)
sp‖Q16539 Mitogen-activated protein kinase 141.0 (0.0)
sp‖P27361 Mitogen-activated protein kinase 3; (and/or MAPK1; Erk 1,2)0.40.02.0 (0.1)
gi‖4885525 NSP1; SH2 domain containing 3A; novel SH2-containing protein 11.11.2 (0.1)0.80 (0.1)1.0 (0.0)
gi‖4502371 NSP2; breast cancer antiestrogen resistance 30.8 (0.1)0.9 (0.0)
sp‖P49023 Paxillin1.00.4
sp‖Q00325 Phosphate carrier protein, mitochondrial precursor (PTP)1.3 (0.1)1.2 (0.1)
sp‖P19174 PLCγ1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase γ0.01.2
sp‖Q99959 Plakophilin 22.7 (0.1)
sp‖P07947 Proto-oncogene tyrosine-protein kinase YES (p61-YES)NC1.0
sp‖P31949 S100 calcium-binding protein A11 (Calgizzarin)1.0
sp‖P29353 SHC transforming protein0.01.1 (0.1)1.0 (0.0)
sp‖P38646 Stress-70 protein (75-kDa glucose regulated)2.0
sp‖Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP)1.2 (0.0)1.0 (0.0)0.9 (0.1)1.1 (0.1)
gi‖17402907 Tripartite motif protein TRIM29 isoform β; ataxia-telangiectasia group D0.8
sp‖P07919 Ubiquinol-cytochrome c reductase complex 11 kDa, mitochondrial0.9 (0.1)1.10.8
sp‖P52735 Vav-2 protein0.0
sp‖P00533 Epidermal growth factor receptor precursor1.0 (0.0)0.2 (0.0)
Phospho-EGFR
sp‖P04626 Receptor protein-tyrosine kinase erbB-2 precursor1.1 (0.0)0.2 (0.0)
Phospho-ErbB2