Table I

Increased proteins during development of basal region in rice seedlings

No.aMMbpIbIdentificationcAmino acid sequencesdHomologous proteinPercentageeAccession no.f
kDa %
Metabolism
    11960.84.9MS(N-blocked)Fatty acid α-oxidase98AF229813
    12160.55.1MS(N-blocked)α-Amylase49X6419
    25350.45.0Ed(N-blocked) I-IHTFNQSQYUTP-glucose-1-phosphate uridyltransferase100Q9SDX3
    38040.86.2MS(N-blocked)Hydrogenase nickel incorporation protein51P26409
    57228.35.3MS(N-blocked)Nitrate reductase20P36858
Energy
    19354.15.1MS(N-blocked)Enolase114AY335488
    41638.45.4Ed(N-blocked) I-KNITXLTRLDMalate dehydrogenase90Q9SML8
    43337.44.5Ed(N-blocked) I-VAFLTQGDAN I-FMFYRNPSADFructokinase100P37829
    46535.25.2MS(N-blocked)NADH-ubiquinone oxidoreductase78AF124786
    50232.57.2EdN-VGPGLYPEIGVoltage-dependent anion channel (OsVDAC1)100Y18104
    54730.24.5Ed(N-blocked) I-AEPTKNF1-ATPase δ-subunit100D88375
Protein synthesis
    10562.84.8MS(N-blocked)Elongation factor Tu89AF303468
    14358.04.6EdN-SAKEIAFDFKBP-type peptidyl-prolyl cis-trans isomerase100Q8E7Q0
    39339.54.5MS(N-blocked)Translation initiation factor 5A46AF094773
    49133.24.3EdN-AVTFTDLHTA I-SGKSSVLLDVElongation factor 1β′100P29545
    49632.84.4EdN-AVTFTDLHTA I-SGKSSVLLDVElongation factor 1β′100P29545
    51831.54.0EdN-TAAEIIKK30 S ribosomal protein S17e75P54026
    60625.64.9EdI-VNIFKDDVVIProbable BTF3b factor protein100A86314
Protein destination and storage
    6169.24.3EdN-EKVVGIDLGTHeat shock protein 70100AY081331
    47734.24.9MS(N-blocked)Heat shock protein 78, mitochondria31P33416
Cell structure
    40938.67.0MS(N-blocked)β-Expansin (EXPB9)36AF261277
Signal transduction
    2179.05.7MS(N-blocked)Benzothiadiazole-induced MAPg kinase 256AY524973
    16156.04.0EdN-EVFFQEKFED I-FSNKDKTLVLCalreticulin precursor100Q9SP22
    57528.14.0Ed(N-blocked) I-SQTIRHMIEDSKP1/ASK-1-like100AY316293
Defense
    57728.14.9EdN-AVLEVVGL I-VTVTMTENTAntiviral protein 2 precursor100Q40772
    62224.56.1EdN-AYVDAAGEEV I-KQHKHKQHLGAbscisic acid stress-inducible protein100AF039573
Unclear function
    1980.05.5MS(N-blocked)O. sativa genomic DNA77AP005201
    6469.25.1MS(N-blocked)ORC4 (origin recognition complex)67AB110983
    10861.94.9MS(N-blocked)NAC6h93AF254558
    53131.03.9MS(N-blocked)Nucleoprotein64P31609
    59726.54.2EdN-MLVYQDLLTG I-GGGDDEGVDDTranslationally controlled tumor protein homolog100P50906
  • a The numbers refer to the spot numbers as given in Fig. 1.

  • b Molecular mass (MM) and pI are from the gel in Fig. 1.

  • c Methods of protein identification: MS and Edman degradation (Ed).

  • d N-terminal (N-) and internal (I-) amino acid sequences as determined by Edman degradation.

  • e The values indicate the sequence coverage for MS and the homology for the identity protein sequences for Edman degradation.

  • f Accession numbers in NCBI data base.

  • g Mitogen-activated protein.

  • h N-acetyl-cysteine 6.