Table II

Decreased proteins during development of basal region in rice seedlings

No.aMMbpIbIdentificationcAmino acid sequencesdHomologous proteinPercentageeAccession no.f
kDa %
Metabolism
    22852.05.3MS(N-blocked)ADP-ribose pyrophosphatase64Q01976
    26549.15.5MS(N-blocked)Cytosolic 6-phosphogluconate dehydrogenase68AF486280
    27148.55.9MS(N-blocked)S-Adenosylmethionine synthetase73AJ296743
    37341.15.5Ed(N-blocked) I-LYNRNDINRIRGP1 (reverse glycosylating protein)100AJ011078
    61425.14.6MS(N-blocked)Methyl-CpG-binding protein78P51608
Energy
    19453.95.0MS(N-blocked)Enolase114AY335488
    27448.36.2MS(N-blocked)Succinate dehydrogenase iron protein49AB017428
    50032.67.5Ed(N-blocked) I-VQYLHPHAGLVoltage-dependent anion channel (OsVDAC2)100AJ251562
    53930.55.5MSAdenylosuccinate synthase29Q97D87
    59027.35.8EdN-GVNEDKYTSDGlyceraldehyde-3-phosphate dehydrogenase100P08735
Transcription
    21052.98.1MS(N-blocked)Transcription factor MADS2144AY177693
Protein synthesis
    31944.85.6MS(N-blocked)60 S ribosomal protein L1968P84098
    34842.85.4MS(N-blocked)60 S ribosomal protein L1968P84098
    38140.74.9MS(N-blocked)50 S ribosomal Protein L3360Q8KCPO
    55829.44.5MS(N-blocked)60 S ribosomal protein L842O13672
    56928.75.5MS(N-blocked)Tyrosyl-tRNA synthetase26O29482
Protein destination and storage
    9563.94.4Ed(N-blocked) I-IIANDQGNRTHeat shock protein100M36115
    18154.74.8EdN-AKELYFNKDG I-NCKLLLVDKKRubisco subunit-binding-protein β subunit100Q43831
    32544.54.4EdN-TAXERAKRTLEquus caballus heat shock protein90AF411802
    33244.04.3EdN-STAQTTIEIDHeat shock protein 70.2100S48024
Cell structure
    60925.45.0EdI-VRTGDYRQLFα-Tubulin100X91807
Signal transduction
    4773.34.5EdN-SDPLFYEPFDCalreticulin family100CAD40786
    15556.95.5EdN-EVFFQEKFCalreticulin precursor100Q9SP22
    31445.05.4MS(N-blocked)Signal transduction 54-kDa protein64Q57565
    48733.34.6Ed(N-blocked)Auxin-responsive protein IAA970Q38827
Defense
    60525.76.5Ed(N-blocked) I-FNVTDIYDAAChitinase III-like protein100BAD31057
    64221.25.7EdN-VKAVVVLGS I-IRHAGDLGNVSuperoxide dismutase100O65768
Secondary metabolism
    57128.65.6EdN-AKNYPVVXAEPutative ascorbate peroxidase90AAO17000
    61125.44.7Ed(N-blocked) I-VSGGPAVPFHHordeum vulgare ascorbate peroxidase80AF411227
Unclear function
    43137.65.2MS(N-blocked)Abnormal long morphology protein 165ALMI-SCHPO
  • a The numbers refer to the spot numbers as given in Fig. 1.

  • b Molecular mass (MM) and pI are from the gel in Fig. 1.

  • c Methods of protein identification: MS and Edman degradation (Ed).

  • d N-terminal (N-) and internal (I-) amino acid sequences as determined by Edman degradation.

  • e The values indicate the sequence coverage for MS and the homology for the identity protein sequences for Edman degradation, respectively.

  • f Accession numbers in NCBI data base.