Table I

Properties of leucine zippers used in this study

pI calculations were made using the site at us.expasy.org/tools/pi_tool.html. Leucine zippers for many of the baits and their in vitro interaction properties were described previously (12–14). N.D., not detectable.

CombinationBaitpIPreyKd for Bait-Prey
m
1EE12345L4.2EE12345LN.D.
2RR12EE345L6.5RR12EE345LN.D.
3EE345.3EE348.1 × 10−4
4RR3410.5RR343.9 × 10−5
5RR1234L11.8RR1234L2.5 × 10−7
6RR3410.5EE341.0 × 10−8
7EE345.3RR341.0 × 10−8
8RR12EE345L6.5EE12RR345L1.3 × 10−11
9EE12RR345L10.4RR12EE345L1.3 × 10−11
10RR12345L12.2EE12345L1.1 × 10−11
11RR1234L11.8EE1234L1.0 × 10−15
12EE1234L4.3RR1234L1.0 × 10−15