Table II

List of protein spots with at least 2.0-fold quantitative alteration in SARS-CoV-infected or uninfected Vero E6 cells based on DIGE analysis

VAMP, vesicle-associated membrane protein; PP2A, protein phosphatase 2A.

DIGE spot no.Protein descriptionGI no.Subcellular locationTheoretical MM (Da)/pIExperimental MM (kDa)/pIPeptide hitsUnique peptidesSequence coverageRatio, DIGE (E6:E6-V)
%
Actin network protein
    42Calponin, acidic isoformgi‖6225157‖Cytoskeletal36,413.61/5.6936.5/5.7712827.051:0.38
Chromosome-associated protein
    20Chromatin assembly factor 1 subunit Cgi‖1172846‖Nuclear47,655.74/4.7455.2/4.714413.181:0.34
Cytoskeletal protein
21α-Tubulin 2gi‖20455316‖Cytoskeletal50,151.71/4.9455.4/5.20151239.691:2.00
Metabolic enzyme protein
    18Pyruvate kinase, M1 isozymegi‖20178296‖No annotation57,913.83/7.9634.3/5.89251019.211:0.37
    19Pyruvate kinase, M1 isozymegi‖20178296‖No annotation57,913.83/7.9634.6/7.63181020.721:0.38
    38Galactokinasegi‖1730187‖No annotation42,272.43/6.0438.9/5.7611613.781:0.32
    43Transaldolasegi‖6648092‖Cytoplasmic37,540.16/6.3636.0/6.345413.351:0.40
    44Pyruvate dehydrogenase E1 component β subunitgi‖129070‖Mitochondrial39,219.39/6.2031.4/5.4318929.251:0.49
Heterogeneous nuclear ribonucleoprotein
    32hnRNP Fgi‖1710628‖Nuclear45,671.98/5.3847.6/5.22161033.011:2.28
Intracellular trafficking protein
    12Transmembrane protein (63 kDa)gi‖19920317‖Membrane66,022.66/5.6362.0/4.767715.611:0.27
    13Transmembrane protein (63 kDa)gi‖19920317‖Membrane66,022.66/5.6361.7/4.785410.301:0.34
    52VAMP-associated protein B/Cgi‖24638339‖Membrane27,228.36/6.8527.3/7.495521.811:0.49
Protein processing protein
    29Mitochondrial processing peptidase β subunitgi‖29840827‖Membrane54,366.18/6.3848.4/5.849511.861:0.40
Signaling proteinActin network protein
    14PP2A subunit A, PR65-α isoformgi‖231443‖No annotation65,223.6/4.9659.8/4.897611.041:0.31
    4714-3-3 protein τgi‖112690‖Cytoplasmic27,764.29/4.6827.5/6.52328.981:2.51
    48Annexin IVgi‖1703319‖No annotation35,882.74/5.8428.0/5.479620.061:0.27
    55Growth factor receptor-bound protein 2gi‖121603‖No annotation25,206.38/5.8925.2/5.9011831.801:0.28