Table IV

List of the proteins or protein spots identified and quantitated in SARS-CoV-infected or uninfected Vero E6 cells by both ICAT and DIGE analysis

Protein descriptionGI no.Subcellular locationTheoretical MM (Da)/pIDIGE spot no.Experimental MM (kDa)/pIPeptide hitsUnique peptidesSequence coverageRatio
ICAT (L:H; E6:E6-V)DIGE (E6:E6-V)
%
Actin network protein
Cofilin, non-muscle isoformgi‖116848‖Cytoplasmic and nuclear18,502.47/8.22606119.0/8.3019.1/6.8214 1120213515.668.4356.121:1.59 ± 0.081:1.811:0:85
Chromosome-associated protein
Chromatin assemblygi‖1172846‖Nuclear47,655.74/4.74113.761:1.63
factor 1 subunit C2055.2/4.714413.181:0.34
Cytoskeletal protein
α-Tubulin 2gi‖20455316‖Cytoskeletal49,959.62/4.98228.221:1.90 ± 0.10
2155.4/5.20151239.691:2.00
Heat shock protein
HSP 90-βgi‖17865718‖Cytoplasmic83,264.38/4.97112.621:1.23
682.5/5.31542434.531:1.51
582.9/5.27201426.801:1.32
Heat shock cognate 71-kDa proteingi‖123648‖No annotation70,897.96/5.37713.411:1.34
970.8/5.78131019.041:0.52
1170.4/4.18151121.051:0.54
1071.0/4.00331930.651:0.72
Hsc70-interacting proteingi‖6686278‖Cytoplasmic41,331.83/5.18314.611:1.41
3148.0/4.9913715.991:1.73
Stress-induced phosphoprotein 1gi‖400042‖No annotation62,639.39/6.40212.581:2.18
1563.4/7.35813046.781:1.22
Heterogeneous nuclear ribonucleoprotein
hnRNP Hgi‖1710632‖Nuclear49,229.59/5.89114.231:1.57
2848.9/6.04291233.411:1.68
2751.0/5.83191134.971:1.60
2651.0/5.83491230.961:1.46
Metabolic enzyme protein
Nucleoside diphosphate kinase Bgi‖127983‖Cytoplasmic, and nuclear17,298.03/8.526213.3/7.92 12115.925.921:1.051:0.57
α enolasegi‖119339‖Cytoplasmic47,168.91/7.0119311.981:1.48 ± 0.08
3347.2/7.001362550.001:1.40
3447.4/7.671882046.081:1.25
Pyruvate kinase, M1 isozymegi‖20178296‖No annotation57,936.87/7.9617511.301:1.75 ± 0.44
2257.5/8.211203556.121:1.42
1758.0/8.009712.431:1.22
1834.3/5.89251019.211:0.37
1934.6/7.63181020.721:0.38
Thioredoxingi‖135773‖No annotation11,737.48/4.82518.571:1.77
6312.0/4.807527.621:1.28
UDP-glucose 6-dehydrogenasegi‖6175086‖No annotation55,024.12/6.73113.041:3.56
2356.6/8.08141329.351:1.27
Signaling protein
14-3-3 protein τgi‖112690‖Cytoplasmic27,764.29/4.68314.491:1.57
4727.5/6.52328.981:2.51
4627.8/4.60215.711:1.29
Annexin IVgi‖1703319‖No annotation35,882.74/5.84527.521:1.62 ± 0.19
4828.0/5.479620.061:0.27