Table III

Result from 20 E. coli samples analyzed regarding the effect of charge state on Mascot score for the ECD/CAD approach (complementary pairs), CADIS, and CAD

The majority of identified peptides were doubly charged (84–91%), and they yield on average a higher Mascot score.

Charge stateNumber of peptidesPercentageAverage Mascot score
Complementary pairs
    2479590.631.9
    34769.022.6
    4170.315.6
    520.0410
    610.022
CADIS
    2484284.520.1
    382914.59.8
    4540.95.1
    510.021
    620.032.5
CAD
    2477987.117.0
    367112.27.9
    4310.64.7
    510.023
    630.051.7