Table II

Myoblast-specific proteins

Listed are representative proteins detected exclusively in the undifferentiated myoblast cell population (MB; day 0) that map to statistically enriched functional categories. The respective GO terms, corresponding Swiss-Prot/TrEMBL locus IDs, protein descriptions, and number of detected spectra are reported. 2–10 days, differentiation time points. ND, not detected.

Functional categories (GO terms) and proteins identifiedMB2 days4 days6 days10 daysDescription
Nucleus (GO:0005634)
    CBP_MOUSE2NDNDNDNDCREB-binding protein
    CUL3_MOUSE2NDNDNDNDCullin homolog 3
    DPO2_MOUSE1NDNDNDNDDNA polymerase α 70-kDa subunit
    NPL1_MOUSE4NDNDNDNDNucleosome assembly protein 1-like 1
Cell cycle (GO:0007049)
    CKS1_HUMANa2NDNDNDNDCyclin-dependent kinases regulatory subunit 1
    CUL3_MOUSE2NDNDNDNDCullin homolog 3
DNA replication (GO:0006260)
    CAFA_MOUSE1NDNDNDNDChromatin assembly factor 1 subunit A
    MCM4_MOUSE2NDNDNDNDDNA replication licensing factor MCM4
    PRI2_MOUSE1NDNDNDNDDNA primase large subunit
    RFA2_MOUSE2NDNDNDNDReplication protein A 32-kDa subunit
Chromosome (GO:0005694)
    HMG4_MOUSE1NDNDNDNDHigh mobility group protein 4
    PR4B_MOUSE1NDNDNDNDSerine/threonine protein kinase PRP4 homolog
    TRF2_MOUSE1NDNDNDNDTelomeric repeat binding factor 2
DNA binding (GO:0003677)
    HCC1_MOUSE3NDNDNDNDNuclear protein Hcc-1
    KF4A_MOUSE3NDNDNDNDChromosome-associated kinesin KIF4A
    RB56_HUMANa2NDNDNDNDTATA-binding protein-associated factor 2N
Protein biosynthesis (GO:0006412)
    IF37_MOUSE5NDNDNDNDEukaryotic translation initiation factor 3 subunit 7
    Q624481NDNDNDNDEukaryotic translation initiation factor 4 γ2
    Q8R3M73NDNDNDNDSimilar to eukaryotic translation initiation factor 3, subunit 8
    R35A_MOUSE1NDNDNDND60 S ribosomal protein L35a
    RL32_HUMANa3NDNDNDND60 S ribosomal protein L32
    MCA1_MOUSE1NDNDNDNDMultisynthetase complex auxiliary component p43
Cytoskeleton (GO:0005856)
    ACTZ_HUMANa6NDNDNDNDα-Centractin (centractin)
    DCT2_MOUSE2NDNDNDNDDynactin complex 50-kDa subunit
  • a Putative mouse homolog detected.