Table IV

Proteins up-regulated in response to myogenic differentiation

Listed are representative proteins mapping to discrete functional categories significantly up-regulated during myotube formation (days 2–10) as compared with undifferentiated myoblasts (MB). ND, not detected.

Functional categories (GO terms) and proteins identifiedMB2 days4 days6 days10 daysDescription
Nucleus (GO:0005634)
    DD19_MOUSEND212NDATP-dependent RNA helicase DDX19
    EMD_MOUSENDNDNDND1Emerin
    GABB_MOUSEND12NDNDGA-binding protein β-1 chain
    HTF4_MOUSEND211NDTranscription factor 12
    ITF2_MOUSENDNDND1NDTranscription factor 4
    LAM1_MOUSENDNDNDND3Lamin B1
    SIX1_MOUSEND113NDHomeobox protein SIX1
    SIX4_MOUSENDND12NDHomeobox protein SIX4
Mitochondrion (GO:0005739)
    ACDL_MOUSENDNDNDND3Acyl-CoA dehydrogenase, long chain-specific
    ACDS_MOUSENDNDNDND2Acyl-CoA dehydrogenase, short chain-specific
    ACDV_MOUSENDNDNDND1Acyl-CoA dehydrogenase, very long chain-specific
    ATPA_MOUSE153817ATP synthase α chain
    ATPB_MOUSE5873159ATP synthase β chain
    ATPD_MOUSENDNDNDND7ATP synthase D chain
    ATPG_MOUSENDNDNDND3ATP synthase γ chain
    COXE_MOUSEND112NDCytochrome-c oxidase polypeptide VIa-liver
    FUMH_MOUSENDNDNDND2Fumarate hydratase, mitochondrial precursor
    IDHG_MOUSEND1NDNDNDIsocitrate dehydrogenase
    MDHM_MOUSE712911Malate dehydrogenase
    NNTM_MOUSENDNDNDND2NAD(P) transhydrogenase
    SODM_MOUSE14594Superoxide dismutase
    UCR2_MOUSENDNDND11Ubiquinol-cytochrome-c reductase complex core protein 2
Calcium ion binding (GO:0005509)
    ATA2_MOUSENDND1NDNDSarcoplasmic/endoplasmic reticulum calcium ATPase 2
    ATA3_MOUSENDNDND13Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
    CALX_MOUSE251096Calnexin precursor
    CAQ1_MOUSENDNDNDND3Calsequestrin, skeletal muscle isoform precursor
    CAQ2_MOUSENDNDND1NDCalsequestrin, cardiac muscle isoform precursor
    FBL1_MOUSEND3NDNDNDFibulin-1 precursor
    FBL2_MOUSE224316Fibulin-2 precursor
    KPCA_MOUSEND232NDProtein kinase C, α type
    NID2_MOUSE141217Nidogen-2 precursor
    NIDO_MOUSEND2NDND2Nidogen precursor
Cell adhesion (GO:0007155)
    CADD_MOUSENDNDNDND3Cadherin-13 precursor
    FINC_MOUSE2366117246Fibronectin
    ITA7_MOUSENDNDNDND3Integrin α7 precursor
    ITA5_MOUSE34265Integrin α5
    ITAV_MOUSENDNDNDND1Integrin αV precursor
Cytoskeleton (GO:0005856)
    DAG1_MOUSEND1NDND4Dystroglycan precursor
    FAK1_MOUSENDNDND1NDFocal adhesion kinase 1
    PLAK_MOUSEND1NDNDNDJunction plakoglobin
    SGCA_MOUSENDNDNDND1α-Sarcoglycan precursor
    SGCB_MOUSEND1ND13β-Sarcoglycan
    SNA1_MOUSENDNDNDND1α1-Syntrophin
    VIME_MOUSE27383254126Vimentin
Muscle development (GO:0007517)
    ACTS_HUMANNDND221Actin, α skeletal muscle
    DESM_MOUSE3171138Desmin
    MLE1_MOUSEND1236Myosin light chain 1, skeletal muscle isoform
    MYH6_MOUSEND5445Myosin heavy chain, cardiac muscle α isoform
    MYHB_MOUSE43416Myosin heavy chain, smooth muscle isoform
    MYPH_MOUSENDND42NDMyosin-binding protein H
    TPCC_MOUSENDNDNDND2Troponin C, slow skeletal and cardiac muscles
    TRT2_MOUSENDND112Troponin T, cardiac muscle isoforms
    KCRM_MOUSENDNDND21Creatine kinase, M chain