Table I

Identification of tryptic fragments of HAF

HAF tryptic fragments shown in Fig. 1 were identified by using the Mascot search engine. P signals were obtained from m-pTGF-β, and B signals were obtained from m-bik. The N-terminal sequences of m-bik and m-pTGF-β as shown in Table II are bold.

Peptide no.RangeAmino acid sequence[M + H]+Mass accuracy
CalculatedObserved
ppm
m-bik
    B11–3 VTK 347.23
    B24–4 K 147.11
    B35–23EDSCQLGYSAGPCMGMTSR2,106.832,106.830
    B424–52YFYNGTSMACETFQYGGCMGNGNNFVTEK3,347.35
    B553–59ECLQTCR966.41966.42+10
    B6a60–70TVAACNLPIVR1,213.671,213.69+16
    B771–74GPCR489.22
    B875–86AFIQLWAFDAVK1,408.76
    B987–88GK204.13
    B1089–103CVLFPYGGCQGNGNK1,670.741,670.71−18
    B11104–108FYSEK673.32
    B12109–111ECR464.19
    B13a112–126EYCGVPGDGDEELLR1,708.751,708.77+12
    B14127–129FSN367.16
    B2b B34–23KEDSCQLGYSAGPCMGMTSR2,234.932,234.92−4
    B11b B12a104–111FYSEKECR1,118.491,118.51+18
m-pTGF-β
    P11–2 AR 246.16
    P23–13NGDHCPLGPGR1,179.53
    P314–16CCR495.18
    P417–21LHTVR625.38625.37c+16
    P5a22–37ASLEDLGWADWVLSPR1,814.911,814.90−6
    P6a38–53EVQVTMCIGACPSQFR1,882.871,882.89+11
    P754–62AANMHAQIK983.51983.50−10
    P863–67TSLHR613.34613.31c−49
    P9a68–91LKPDTVPAPCCVPASYNPMVLIQK2,698.342,698.35+4
    P10a92–107TDTGVSLQTYDDLLAK1,739.871,739.88+6
    P11108–112DCHCI704.25704.26c+14
    P10b P1192–112TDTGVSLQTYDDLLAKDCHCI2,425.102,425.09−4
  • a Tryptic peptides were also observed in the 27-kDa gel band from non-reducing SDS-polyacrylamide gels (Fig. 1D).

  • b Indicates a missed cleavage.

  • c Low molecular weight ions not shown in Fig. 1.