Table I

MRM transition masses along with optimal collision energies calculated for the Cdc2 tryptic peptide IGEGTYGVVYK with two potential modifications and a charge state of +2 or +3 selected

Q1 massQ3 massAmino acid sequenceFragment typeFragment massChargeCollision energy
Da eV
673.2624.2IGEGpTpYGVVYKLoss of98.0+224.1
673.2216.0IGEGTpYGVVpYKFragment216.0+260.0
633.2584.2IGEGpTYGVVYKLoss of98.0+222.3
633.2216.0IGEGTpYGVVYKFragment216.0+260.0
449.1416.5IGEGpTpYGVVYKLoss of98.0+316.8
449.1216.0IGEGTpYGVVpYKFragment216.0+360.0
422.5389.8IGEGpTYGVVYKLoss of98.0+316.1
422.5216.0IGEGTpYGVVYKFragment216.0+360.0