Table III

Quantitation of MMS-induced changes in phosphorylationa

All identified peptides are tryptic. The amino acid preceding the N terminus of the peptide is indicated in parenthesis with the exception of N-terminal peptide of the protein.

ProteinPeptideMMS/CTLbCTLMMSPhosphorylation site
Rad53(K)IAS*PGLTSSTASSMVANK1.7XcX175
(K)DTSVS*PDEYEER2.7XX375
(K)KPPVSDTNNNGNNSVLNDLVES*PINANTGNILK2.8XX774
(K)MSPLGSQSYGDFSQISLSQSLS*QQK3.0XX489
(R)GKD(TS)*VSPDEYEER4.0X(372–373)
(R)DLS*ADISQVLK5.0X49
(R)IHS*VSLSQSQIDPSK6.8XX789
(K)FS*QEQIGENIVCR12.0XX24
(K)IPAHAPIRYT*QPK12.5X543
(R)IHS*VSLS*QSQIDPSK12.5X789/793
(K)VQGNGS*FMK14.3X350
(K)LLHS*NNTENVK14.3XX560
(K)QIGRGS*YHEGPLKDFR14.3X424
(K)S*IEAETR14.5X547
(R)ANQPSASS(SS)*MSAK59.0X(747–748)
Rad9(R)KSMTNVLS*PK3.5X937
(K)EALHS*PLADGDMNEMNVPVDPLENK3.9X26
(R)INFEPILEVPETSS*PSK6.7X494
(R)NNQIIQ(SNES)*EEINELEK7.3X(312–315)
(K)RNS*DLDAASIKR7.9X729
(R)S*ADNYDCALEGIVTPK7.9X205
(K)NLMQLFPSES*QEIIQNR8.1X688
(K)SS*PDRVTQSAIK8.1X11
(K)VNSTNIIEGS*PK8.2X56
(R)LSLDS*PSK8.4X1136
(R)DALAEHAGAPSLLF(SS)*GEIR8.6X(989–990)
(K)NLNVS*GR16.0X328
Asf1(K)IEGGSTDIEST*PK3.3XX270
Yta7(K)EQS*QENILQEPDLK7.5X1142
(R)GGWNAS*QNSGPTR14.9X316
(K)ENEDS*LQTQVTEENFSK22.0X1256
Nup2(R)ETYD(SNES)*DDDVTPSTK7.2X(17–20)
  • a Each asterisk indicates one phosphorylation event to the S/T residue to its left. In cases where the exact site is ambiguous due to clustering of multiple S/T residues, the possible candidate S/T residues are shown in parentheses.

  • b Value for peptides identified only in MMS-treated samples should be considered as a signal-to-noise ratio.

  • c X indicated the presence of the phosphopeptides in the indicated sample.