Table II

Results of the analysis of E. coli peptides by OFFGEL electrophoresis and HPLC-Chip/MS

The expected pH ranges per fraction were calculated taking into account the OFFGEL well dimensions and the specifications of the IPG gel strip supplier. Average pI values were calculated from all peptides identified in each well.

FractionExpected pH rangeUnique/total peptidesAverage pI with standard deviation
13.35–3.6143/973.85 ± 0.19
23.61–3.88343/4733.99 ± 0.18
33.88–4.14421/5534.19 ± 0.17
44.14–4.40372/4864.38 ± 0.13
54.40–4.66216/3164.58 ± 0.14
64.66–4.9381/1434.71 ± 0.17
74.93–5.1927/605.08 ± 0.45
85.19–5.454/85.07 ± 0.58
95.45–5.7167/2485.66 ± 0.31
105.17–5.9887/3375.73 ± 0.25
115.98–6.24114/3885.89 ± 0.18
126.24–6.5076/3445.98 ± 0.15
136.50–6.7637/2416.06 ± 0.46
146.76–7.0331/1446.15 ± 0.43
157.03–7.2916/1056.39 ± 0.58
167.29–7.5532/1186.62 ± 0.55
177.55–7.8146/916.74 ± 0.28
187.81–8.083/167.12 ± 0.84
198.08–8.342/178.00 ± 1.49
208.34–8.603/428.56 ± 0.56
218.60–8.8620/1338.68 ± 0.45
228.86–9.1342/2208.69 ± 0.45
239.13–9.3979/2898.85 ± 0.53
249.39–9.65104/2519.14 ± 0.63