Table I

Functional classification and abundance of the 241 identified proteins in the chloroplast stroma on the CN-PAGE gels

aa, amino acid; CHO, carbohydrates; OPPP, oxidative pentose phosphate pathway.

MapMan BinsaFunctional classificationbNumber identifiedNumber (percentage of total)Relative amount of protein massc
%
1.1Thylakoid93.70.8
1.2, 1.3, 1.4, 1.7Calvin cycle, OPPP, glycolysis3012.475.8
2, 3Minor and major CHO125.00.4
8Organic transformation62.51.4
12, 14Nitrogen, sulfur assimilation41.77.6
13Amino acid synthesis and degradation177.10.8
11, 16, 17, 18Lipid, hormone, isoprene, vitamin, and cofactor synthesis104.11.1
19Tetrapyrroles145.80.5
20, 21Redox and stress208.31.9
23Nucleotide synthesis and degradation104.10.5
27RNA72.90.6
29.1 and 29.2Protein synthesis2912.01.4
29.3–29.9Protein targeting, folding, and degradation3414.15.2
24, 26, 28, 35Unknown and miscellaneous3916.21.7
  • a Functional assignment according to the MapMan Bin classification (29) and reported in PPDB.

  • b Summarizing functional classification.

  • c Relative protein amount for all proteins in this functional class normalized to the total amount on the CN-PAGE gel.