Table I

Proteins secreted by osteoclasts captured on an MPR affinity column

ProteinNCBI gene identifierPredicted molecular massNo. of sequenced peptidesMan-6-PPropeptide sequence (position, MS/MS score)Change in mRNA expressionaBone phenotype (syndrome)
Da
Peptidases
    Dipeptidyl-peptidase I302345452,34310bYes149VNMNAAHLGGLQER162 (89), 167LYTHNHNFVK176 (42), 184SWTATAYK191 (35), 184SWTATAYKEYEK195 (35)b−2.7Yes (Papillon-Lefevre, Haim-Munk)
    Dipeptidyl-peptidase II1362639056,2349b1.3
    Tripeptidyl-peptidase I1264408561,30412b, cYes183QRPEPQQVGTVSLHLGVT-PSVLR205 (44)c1.3
    Retinoid-inducible serine carboxypeptidase4847458650,93413b−1.9
    Angiotensinase C-like3346901555,7641b, d
    Cathepsin A13108253,80913b, cYesYes (Goldberg)
    γ-Glutamyl hydrolase1312425635,4159b−2.3
    Cathepsin Z1258520933,97410b, c31SGQTCYHPIRGDQLALL-GR49 (45), 51TYPRPHEYLSPADLP-K66 (45)c
    Cathepsin B11571237,25611b, cYes72VAFGEDIDLPETFDAR87 (108)c−1.8
    Cathepsin L11574237,52312bYes21FDQTFSAEWHQWK33 (81), 38RLYGTNEEEWR48 (32)c−1.8Yes
    Cathepsin S1264331838,4136b, cYes29DPTLDYHWDLWK40 (48), 29DPTLDYHWDLWKK41 (54), 49DKNEEEVR56 (49)b−2.2
    Legumain2161782149,34112b, c324TNDVKESQNLIGQIQQFLD-AR344 (80), 329ESQNLIGQIQQFLDAR344 (51), 354IVSLLAGFGETAER367 (59)c−3.8
    Cathepsin K1264432036,8658bYes36QYNSKVDEISR46 (30)b8.4Yes
    Cathepsin F1264332151,6286b47FALDMYNYGR56 (64), 244SINDLAPPEWDWR256 (72)b1.6
    Cathepsin D11571844,92512b, cYes35TMTEVGGSVEDLILKGPITK54 (52)c−1.4
Glycosidases
    Di-N-acetylchitobiase2722920441,5042b−1.6
    Sialidase 11736796744,5635bYesYes
    Lysosomal α-glucosidase51338793106,18010bYes
    α-Galactosidase A170321047,6111b, eYes
    β-Galactosidase11494473,07414bYes3.2Yes (Morquio)
    Lysosomal α-mannosidase17380364114,53234b, cYes−1.6Yes
    Epididymis-specific α-mannosidase17367999115,55131b, c−1.8Yes
    β-Mannosidase13310141101,3202bYes1.6Yes
    β-Glucuronidase11496474,19223b, cYesYes
    β-Glucosidase12128457,5852bNoYes (Gaucher)
    α-N-Acetylglucosaminidase730529982,1154bYes−1.3Yes (Sanfilippo)
    Tissue α-l-fucosidase3154178153,4522bYes−1.5
    β-Hexosaminidase α chain23225560,56016bYes37YTLYPNNFQFR47 (47)c
    β-Hexosaminidase β chain134628061,07720b, cYes31LQPALWPFPR40 (47)c1.8
    α-l-Iduronidase135242471,13511bYesYes (Hurler)
    N4-(β-N-Acetylglucosaminyl)-l-asparaginase249816336,9985bYes
Sulfatases
    Galactosamine (N-acetyl)-6-sulfatase3198065457,9894bYesYes (Morquio)
    Arylsulfatase A170342053,7424bYes
    Arylsulfatase B3330260142,8685bYesYes (Maroteaux-Lamy)
    Glucosamine (N-acetyl)-6-sulfatase2978923962,0425bYes1.5Yes (Sanfilippo)
Da
    N-Sulfoglucosamine sulfohydrolase3154369756,6592bYesYes (Sanfilippo)
Lipid Metabolism
    Lysosomal phospholipase A24488810747,2775b−1.5
    Lysosomal acid lipase/cholesteryl ester hydrolase2013879845,5218bYes−3.5
    Lipoprotein lipase41725553,0932bNo
    Sialate O-acetylesterase2509174160,7243b
    Palmitoyl-protein thioesterase 12014166234,46710b, cYes−1.4
    Palmitoyl-protein thioesterase 22013887534,3443b
    Acid sphingomyelinase-like phosphodiesterase 3a1820233749,8073b−3.1
    Sphingomyelin phosphodiesterase135198269,8823bYes2.4
    Acid ceramidase813432944,6414bYes
    Epididymal secretory protein E11442383116,4325b, cYes2.4
Others
    Procollagen-lysine,2-oxoglutarate 5-dioxygenase 12500893883,54213bYes (Ehlers-Danlos)
    Tartrate-resistant acid phosphatase type 573035736,7846bYes530
    Deoxyribonuclease IIα318298938,7868b
    Ribonuclease T22013971829,5905b3.5
    Interleukin-4-induced protein 1391365270,1474b
    Dentin matrix protein 45643526364,4052b−2.4
    Prosaposin391493961,38110bYes−1.3
    Interferon γ-inducible protein 301521249227,7942cYes
Possible contaminants
    Lysozyme C, type P12659916,7832b−1.7
    β-1,4-Galactosyltransferase 51312398244,7013b−1.8
    Polypeptide N-acetyl-galactosaminyltransferase 65131598871,4918b1.4
    CMP-N-acetyl-neuraminate-poly-α-2,8-sialyltransferase249483541,2302b1.4
  • a Average -fold change ratio in Raw 264.7 cells vs. osteoclasts (p < 0.05).

  • b Peptide sequencing by LC-ESI-Q-TOF.

  • c Peptide sequencing by MALDI-TOF/TOF.

  • d Precursor ion, 537.27[M + 2H]2+; sequence, K113AMLVFAEHR121Y; score, 72.

  • e Precursor ion, 545.30[M + 2H]2+; sequence, R407VNPSGTVLFR416L; score, 51.