Table I

Protein coverage from the LCMSE analysis of the six-protein mixture

These results reflect those peptides that replicated in at least two of the three replicate injections.

DescriptionMolecular weightPercent protein coverage, number of peptides, concentrationa
Sample 1Sample 2Sample 3Sample 4Sample 5Sample 6
Enolase46,62477, 32, 15.0062, 29, 7.5061, 27, 3.0051, 23, 1.5037, 17, 0.7522, 11, 0.30
Serum albumin69,24879, 55, 12.5079, 54, 6.2577, 53, 2.5069, 47, 1.2549, 28, 0.6331, 18, 0.25
Alcohol dehydrogenase I36,66972, 27, 10.0071, 26, 5.0071, 26, 2.0047, 20, 1.0031, 12, 0.5025, 8, 0.25
Phosphorylase B97,09769, 62, 6.0067, 60, 3.0055, 49, 1.2034, 32, 0.6014, 12, 0.302, 2, 0.12
Hemoglobin (α)15,04484, 9, 5.0084, 9, 2.5084, 9, 1.0059, 7, 0.5045, 6, 0.2510, 1, 0.10
Hemoglobin (β)15,94491, 14, 5.0082, 12, 2.5082, 12, 1.0071, 10, 0.5039, 5, 0.258, 1, 0.10
  • a Concentration is reported as picomoles loaded onto a 300-μm column.