Table II

Identified peptides to P02070 bovine hemoglobin (β chain) (5 pmol) obtained from the LCMSE analysis of the stock protein mixture in Table I

The table includes the average mass, retention time, and intensity measurements calculated from the triplicate analysis. The residues in parentheses are the N- (left) and C-terminal (right) residues flanking the tryptic peptide as determined from the intact protein sequence.

MH+aMass RSDRtaRt RSDIntensityaInt RSDActual MH+ΔSequence
ppm
740.39771.010.122.435,79223.8740.39434.6(K)HLDDLK(G)
821.41500.912.353.414,79816.4821.4079−9.3(−)MLTAEEK(A)
950.51611.951.640.746,0478.9950.50996.5(K)AAVTAFWGK(V)
1,097.53460.539.591.422,65612.41,097.53014.0(K)VLDSFSNGMK(H)
1,098.56210.937.001.343,3538.61,098.55833.4(K)LHVDPENFK(L)
1,101.56250.930.261.047,9775.41,101.55417.6(K)VDEVGGEALGR(L)
1,177.67730.437.841.525,34614.11,177.6805−2.7(K)VVAGVANALAHR(Y)
1,265.82873.994.010.762329.71,265.8309−1.8(K)LLGNVLVVVLAR(N)
1,274.72920.972.380.9112,6032.21,274.72612.4(R)LLVVYPWTQR(F)
1,328.71560.834.911.439,27313.11,328.7174−1.4(K)VKVDEVGGEALGR(L)
1,422.73150.861.321.0172,3292.41,422.72693.2(K)EFTPVLQADFQK(V)
1,448.68560.350.321.280,8764.71,448.6844−1.1(K)GTFAALSELHCDK(L)
2,089.96331.177.590.9110,2913.42,089.95414.4(R)FFESFGDLSTADAVMNNPK(V)
2,105.95920.669.591.011,3273.62,105.94904.8(R)FFESFGDLSTADAVM*NNPK(V)
Average1.1b1.3b10.6b4.7c
  • a The relative standard deviation was determined from the replicate mass, retention time, and intensity measurements of each detected peptide. The mass accuracy is reported for each identified peptide. An overall relative standard deviation is reported for the mass RSD, retention time RSD (rt RSD), and intensity RSD (int RSD).

  • b An overall root mean square error is reported for all the identified peptides to bovine beta hemoglobin.

  • c Oxidized methionine is denoted as M*.