Table IV

Summary of the absolute quantification results obtained from the analysis of the six-protein mixture described in Table I in human serum

Alcohol dehydrogenase (bold) was used as the internal reference standard in both studies as discussed in the text.

Relative ratioAverage SR of top three peptidesProteinTheoreticalCalculatedErrorSR/pmol
pmol pmol %
1.36287,764Enolase15.013.6−9.319,184
1.29273,241Serum albumin12.512.93.321,859
1.00 211,572 Alcohol dehydrogenase 10.0 10.0 0.0 21,157
0.65137,933Phosphorylase B6.06.58.722,989
0.47100,208Hemoglobin (β)5.04.7−5.320,042
0.4391,745Hemoglobin (α)5.04.3−13.318,349
211,572NormalizationAverage SR/pmol20,597
Cv8.4