Table V

Absolute quantification of human serum proteins

ProteinkDaIntensityapmolbngcμg/μldpmol/mlepg/mllog10 (pg/ml)
Albumin70.01,896,53092.086,445.4651.56736,62451,563,664,61110.7
α2-Macroglobulin163.066,8013.24528.654.2325,9464,229,184,0569.6
Transferrin77.0113,4855.51424.253.3944,0783,394,026,3159.5
C3 complement187.035,5931.73323.152.5913,8252,585,188,5239.4
Apolipoprotein A-I31.0146,2747.10220.151.7656,8141,761,225,0339.2
IgG total36.0107,7145.23188.271.5141,8371,506,123,8049.2
Haptoglobin38.077,9943.79143.891.1530,2931,151,147,0609.1
α1-Antitrypsin47.033,5011.6376.450.6113,012611,563,6268.8
Ceruloplasmin122.010,7890.5263.910.514,191511,242,6088.7
IgM total49.525,0641.2260.240.489,735481,882,9938.7
α1-Acid glycoprotein23.538,4201.8743.840.3514,923350,680,1968.5
  • a Average signal response of the three most intense tryptic peptides.

  • b The calculated mole quantity of protein loaded onto the analytical column (pmol).

  • c The calculated mass quantity of protein loaded onto the analytical column (ng).

  • d The calculated concentration of protein in the original sample (μg/ml).

  • e The calculated concentration of protein in the original sample (pmol/ml).