Table I

Partial list of proteins identified to be up-regulated in pancreatic cancer (Panc1) versus non-neoplastic (HPDE) cells by SILAC

RefSeq accession no.Protein nameRatio (Panc1/HPDE)LocalizationSignal peptideProtein functionPreviously reported in pancreatic cancer/Ref.
SILACDNA array
NP_001141.1 Aminopeptidase N20a0.7Extracellular, plasma membraneYesMetalloprotease, protein metabolism 102
NP_002264.1 Keratin 820a2.5CytoplasmNoStructural protein, cell growth 10
NP_006175.2 Nucleobindin 120a0.3Extracellular, GolgiYesCalcium-binding protein, signal transduction 103
NP_057631.1 SDF420a1Golgi, ERYesCalcium-binding protein, signal transductionNo
NP_000032.1 Apolipoprotein E20a0.9ExtracellularYesTransport/cargo proteinNo
NP_839946.1 Hyaluronan20aTNPbExtracellularYesAdhesion molecule, signal transduction104–106
NP_002973.1 MCP-120a270ExtracellularYesChemokine, immune response107–109
NP_039268.1 Dimethylarginine dimethylaminohydrolase 220a4.2CytoplasmNoHydrolase, metabolismNo
NP_002309.1 Lysyl oxidase-like 220aTNDcExtracellularYesOxidase, cell growthNo
NP_060599.1 Pyridoxine 5′-phosphate oxidase20a0.5CytoplasmNoOxidase, metabolismNo
XP_291627.3 Prostate cancer-associated gene 520aTNPUnknownNoUnknown 37
NP_002769.1 Saposin A10.71ExtracellularYesIntegral membrane protein, signal transduction 103
NP_001760.1 CD9 antigen8.00.5Plasma membraneNoImmune response 10
NP_001900.1 Cathepsin D6.71.2Lysosome, extracellularYesAspartic protease, protein metabolism10, 100, 110
NP_006817.1 Tryptophan 5, monooxygenase activation protein (14-3-3 θ)6.21.7CytoplasmNoAdapter molecule, signal transductionNo
NP_001279.2 Chloride intracellular channel 15.62.8Plasma membrane, nucleusNoLigand-gated channel, transportNo
NP_002061.1 Guanine nucleotide-binding protein5.41Plasma membrane, ERNoGTPase, signal transductionNo
NP_001869.1 Cellular retinoic acid-binding protein 25.30.9CytoplasmNoTranscription regulatory proteinNo
NP_004915.2 Actinin, α 45.23.8CytoplasmNoCytoskeletal protein, cell growthNo
NP_000416.1 L1CAM5.212Plasma membraneYesAdhesion moleculeNo
NP_005520.2 Perlecan (HSPG2)5.10.8ExtracellularYesExtracellular matrix protein, cell growthNo
NP_066953.1 Peptidylprolyl isomerase A (cyclophilin A)4.91CytoplasmNoChaperone, protein metabolism100
NP_005558.1 Galectin-3-binding protein (Mac-2BP)4.90.5ExtracellularYesExtracellular matrix protein, immune response44, 111
NP_004379.1 Di-N-acetylchitobiase4.72.8LysosomeYesGlycosidase, metabolismNo
NP_001366.1 Deoxyribonuclease II4.70.9LysosomeYesDeoxyribonuclease, metabolism 112
NP_690619.1 PTK74.60.8Plasma membraneYesTyrosine kinase, signal transductionNo
NP_066012.1 KIAA16274.5TNPUnknownNoMethyltransferase, metabolismNo
NP_003364.1 Vinculin4.3TNDCytoplasmNoCytoskeletal protein, cell growthNo
NP_055703.1 Predicted osteoblast protein (FAM3C)4.21.1ExtracellularYesCytokine, signal transductionNo
NP_001055.1 Transketolase4.21CytoplasmNoTransketolase, metabolismNo
NP_001091.1 Actin, α 14TNDCytoplasmNoStructural protein, cell growthNo
NP_002863.1 Ras-related C3 botulinum toxin substrate 2 (Rac-2)3.5TNDPlasma membraneNoGTPase, signal transductionNo
NP_002202.2 Fibronectin receptor(integrin β1)3.41.2Plasma membrane, ER, extracellularYesCell surface receptor, signal transduction73, 113
NP_000475.1 Amyloid β (A4)30.4Plasma membrane, ER, extracellularYesCell surface receptor, signal transduction 114
NP_006073.1 Tubulin, α32CytoplasmNoStructural protein, cell growth 10
NP_478059.1 Phosphoserine aminotransferase isoform 130.5ExtracellularNoAminotransferase, metabolismNo
  • a An arbitrary value of 20 was chosen for proteins where the intensity of the peptides in the MS spectra was either very low or absent in normal (HPDE) compared to cancer (Panc1).

  • b TNP, transcript not present on the chip.

  • c TND, transcript not detected on the chip.