Table I

Yeast proteins that interact with the five polyanion probes

The information provided in the table is extracted from the SGD at db.yeastgenome.org/cgi-bin/GO/goEvidence.pl (see also ST 1). IEP, inferred from expression pattern; ND, no biological data available; TAS, traceable author statement; IDA, inferred from direct assay; IGI, inferred from genetic interaction; IMP, inferred from mutant phenotype; IPI, inferred from physical interaction; ISS, inferred from sequence or structural similarity.

ORFNameLocationBiological processMolecular function
YBR031WRibosomal protein L4A (L2A) (rp2) (YL2)Cytosolic large ribosomal subunit (sensu Eukaryotae) (TAS)Protein biosynthesis (TAS)Structural constituent of ribosome (TAS)
YBR118WTranslational elongation factor EF-1αEukaryotic translation elongation factor 1 complex (63), ribosome (TAS)Translational elongation (TAS)Translation elongation factor activity (TAS)
YCR016WHypothetical proteinNucleolus (IDA), nucleus (IDA)UnknownUnknown
YDL051WLa homologous proteinNucleolus (IDA), nucleoplasm (IDA), nucleus (IDA)tRNA processing (IMP)RNA binding (IDA)
YDL078CMalate dehydrogenasePeroxisomal matrix (TAS), peroxisome (IDA)NADH regeneration (TAS), fatty acid β-oxidation (TAS),t…l-Malate dehydrogenase activity (IMP, TAS)
YGR026WUnknownUnknownUnknownUnknown
YHL033CRibosomal protein L8A (rp6) (YL5) (L4A)Cytosolic large ribosomal subunit (sensu Eukaryotae) (TAS)Protein biosynthesis (TAS)Structural constituent of ribosome (TAS)
YHR117WProtein translocase 71-kDa component of the outer membrane of mitochondriaMitochondrial outer membrane (IDA)UnknownProtein transporter activity (IPI, ISS)
YKR009CMultifunctional β-oxidation protein in fatty acid oxidationPeroxisomal matrix (TAS)Fatty acid β-oxidation (TAS)3-Hydroxyacyl-CoA dehydrogenase activity (TAS), enoyl-CoA hydratase activity (TAS)
YLR003CHypothetical proteinNucleus (IDA)Regulation of DNA replication (IGI)Unknown
YLR150WPurine motif triplex-binding proteinCytoplasm (IDA), cytosolic ribosome (sensu Eukaryotae) (IDA), nuclear telomere cap complex (IGI)Antiapoptosis (IDA), telomere maintenance (IMP)DNA binding (IDA), telomeric DNA binding (TAS)
YMR153WKaryopherin docking complex component of the nuclear pore complex nuclear pore complex subunitNuclear poremRNA binding, protein export from nucleus, …Structural molecule activity
YOR056CEssential nuclear protein involved in proteasome maturation and synthesis of 40 S ribosomal subunits; required for cleavage of the 20 S pre-rRNA to generate the mature 18 S rRNANucleus (IDA), preribosome (IDA, IEP, IPI)Processing of 20 S pre-RNA, proteasome assembly, ribosomal small subunit biogenesisRNA (IDA, ISS) and protein binding (IDA, IMP)
YOR309CHypothetical proteinCellular component unknown (ND)UnknownMolecular function unknown (ND)
YPR086WTranscription factor TFIIB homologNucleoplasmTranscription initiation from RNA polymerase II promoterGeneral RNA polymerase II transcription factor activity
YPR139CVacuolar protein sortingCytoplasmProtein-vacuolar targeting (IMP)Unknown