Table I

Selected 14-3-3ζ-associated proteins identified by TAP-MS from transgenic mouse brains

Proteins were identified through a search of LC-MS/MS data against a combined human and mouse protein database, and the corresponding human proteins are listed according to their unique Human Genome Organisation (HUGO) gene symbols and National Center for Biotechnology Information (NCBI) gene ID. Domain composition obtained from SMART (Simple Modular Architecture Research Tool; dylan.embl-heidelberg.de/) and Pfam (Protein Family Database; pfam.wustl.edu/) is indicated. The absence (0) or the number (1–6) of consensus binding sites (CS) for 14-3-3 proteins are shown. UBA, ubiquitin-associated; RIIa, RIIα, regulatory subunit portion of type II PKA R-subunit; cNMP, cyclic nucleotide monophosphate-binding domain; ADK, adenylate kinase; RBD, ras-binding domain; C1, protein kinase C conserved region 1; STYKc, tyrosine kinase-like domain; PI4Kc, phosphoinositide 4-kinase, catalytic domain; IPK, inositol polyphosphate kinase; SAM, sterile α motif; DENN, differentially expressed in neoplastic versus normal cells; PH, pleckstrin homology domain; SH3, Src homology domain 3; RA, Ras association domain; RasGEF_N, GEF for Ras-like GTPases, N-terminal domain; RasGRF, Ras protein-specific guanine nucleotide-releasing factor domain; C2, protein kinase C conserved region 2; SEC14, Sec14p-like lipid-binding domain; ANK, ankyrin repeats; ArfGap, putative GTPase-activating proteins for the small GTPase ARF; IQ, calmodulin-binding motif; RasGEFN, GEF for Ras-like GTPases, N-terminal motif; VHP, Villin headpiece domain; CAP-Gly, cytoskeleton-associated proteins-Gly domain; KISc, kinesin motor domain; TPR, tetratricopeptide repeat domain; WH1, WASp homology domain 1; Iso_dh, isocitrate/isopropylmalate dehydrogenase; RING, Really Interesting New Gene domain; MIR, domain in ryanodine and inositol trisphosphate receptors and protein O-mannosyltransferases; RYDR_ITPR, RIH (RyR and IP3R homology) domain; BTB_POZ, BTB (for BR-C, ttk, and bab)/POZ (for pox virus and zinc finger) domain; K_tetra, K+ channel tetramerization domain; Aldo_ket_red, aldo/keto reductase family; BRLZ, basic region leucin zipper.

Gene IDGene symbolDescriptionDomainsCS
Signaling kinases
    17169Mark3Microtubule-associated protein/microtubule affinity-regulating kinase 3Kinase domain, UBA domain0
    12322 Camk2a a Calcium/calmodulin-dependent protein kinase (CaM kinase) IIαSer/Thr kinase domain0
    12323Camk2baCalcium/calmodulin-dependent protein kinase (CaM kinase) IIβSer/Thr kinase domain0
    12325Camk2gaCalcium/calmodulin-dependent protein kinase (CaM kinase) IIγSer/Thr kinase domain0
    70661BC033915aSer/Thr protein kinaseS_TKc1
    19088Prkar2bacAMP-dependent protein kinase type II-β regulatory chainRIIa, cNMP0
    229949Ak5aAdenylate kinase 5, ATP-AMP transphosphorylaseADK1
    109880Brafv-raf murine sarcoma viral oncogene homolog B1RBD, C1, STYKc3
    224020 Pik4ca a Similar to phosphatidylinositol 4-kinase αPI4Kc1
    228550ItpkaaInositol 1,4,5-trisphosphate 3-kinase AIPK0
Phosphatases, pseudophosphatases, and phosphodiesterases
    19047Ppp1ccaProtein phosphatase 1, catalytic subunit, γ isoformPP2Ac1
    19052Ppp2caProtein phosphatase 2 (formerly 2A), catalytic subunit, β isoform0
    51792 Ppp2r1a Protein phosphatase 2, regulatory subunit A, α isoformHEAT repeat0
    19057 Ppp3cc a Protein phosphatase 3, catalytic subunit, γ isoform0
    53332Mtmr1aMyotubularin-related protein 1TYR_phosphatase, GRAM0
    233977Ppfia1Protein-tyrosine phosphatase, interacting protein (liprin), α1SAM1
    327814 Ppfia2 a Protein-tyrosine phosphatase, interacting protein (liprin), α2SAM2
    68507Ppfia4aProtein-tyrosine phosphatase, interacting protein (liprin), α4SAM2
    77980Sbf1aSET-binding factorDENN, GRAM, PH2
    18573Pde1aaCalmodulin-dependent phosphodiesterasePDEase0
    18578Pde4baPhosphodiesterase 4BPDEase1
Signaling, small GTPase-related proteins
    16800Arhgef2Rho/Rac GEF2PH, RhoGEF, C11
    54126 Arhgef7 PIXB, Rho GEF7SH3, RhoGEF, PH1
    102098 Arhgef18 a Rho/Rac GEF18; p114-Rho-GEFRhoGEF, PH2
    76089Rapgef2aRap GEF2 = PDZ-GEF1cNMP_binding, PDZ, RA, RasGef_N, RasGRF3
    110279BcraGTPase-activating protein for p21racC2, PH, RhoGAP, RhoGEF1
    18015Nf1aNeurofibromin 1RasGAP, SEC141
    240057Syngap1aSynaptic Ras GTPase-activating protein 1 homologRasGAP, C2, PH6
    216963Git1G protein-coupled receptor kinase interactor 1GIT1, ANK, ArfGap1
    19418 Rasgrf2 a Ras protein-specific guanine nucleotide-releasing factor 2PH, IQ, RhoGEF, RasGEFN, RasGEF2
    228355MaddaMitogen-activated protein kinase-activating death domain, (GDP/GTP exchange protein)dDENN, DENN, uDENN3
    111173Rab6ip2Rab6-interacting protein 2ELKS, Spectrin1
    52055 Rab11fip5 a GAF1, RAB11 family-interacting protein 5C2 domain4
Cytoskeleton-related proteins
    13829Epb4.9aErythrocyte membrane protein band 4.9 (dematin)VHP; Villin headpiece domain2
    269713Cyln2aCytoplasmic linker 2CAP-Gly0
    16573Kif5bKinesin family member 5BKISc domain0
    16574 Kif5c Kinesin family member 5CKISc domain0
    16593 Kns2 Kinesin 2 (60–70 kDa); kinesin light chain 14 TPR repeats0
    16594 Klc2 Likely ortholog of kinesin light chain 2 (FLJ12387)5 TPR repeats1
Signaling adaptor proteins
    7534 YWHAZ 14-3-3ζ
    54401 Ywhab 14-3-3β/α
    22627 Ywhae 14-3-3ε
    22629 Ywhah 14-3-3η
    22628 Ywhag 14-3-3γ
    22630 Ywhaq 14-3-3τ
    26558 Homer3 Regulate group 1 metabotropic glutamate receptor functionWH10
Metabolism, homeostasis
    18640 Pfkfb2 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase 26-Fructo kinase domain1
    13808 Eno3 a Enolase 3; 2-phospho-d-glycerate hydrolyase0
    170718Idh3baNAD+-dependent isocitrate dehydrogenaseIso_dh0
Cellular and vesicle trafficking
    55992Trim3aTripartite motif-containing 3B-box-type zinc finger, RING finger, filamin-type immunoglobulin domains2
    26949Vat1aVesicle amine transport protein 1 homolog0
Ion channel, transport carrier
    16438Itpr1aInositol 1,4,5-trisphosphate receptor, type 1MIR, RYDR_IPTR0
    383348Kctd16aPotassium channel tetramerization domain-containing 16BTB_POZ, K_tetra0
    16498Kcnab2aPotassium voltage-gated channel, β member 2Aldo/ket_red1
Transcription, chromatin structure
    16825Ldb1aLIM domain binding 10
    93760Arid1aaSMARCF1; chromatin-remodeling factor p2502
    20587Smarcb1aSWI/SNF-related, SNF5 homologSNF5 domain2
Miscellaneous
    108686A430106J12RikaPeptidase activityBRLZ2
    66049RogdiaLeucine zipper domain proteinLeucine zipper domain0
  • a Protein specifically interacting in brain.