Table I

S-Glutathiolated proteins following biotin-GSSG treatment

Proteins purified following biotin-GSSG treatment were identified using LC-MS/MS. The peptide sequences and MASCOT score used to identify the proteins are also shown. The residues shown in parentheses are amino acid residues that we did not actually determine but can be deduced from database sequences. We have included them to illustrate that all the peptides used to make identifications are bona fide tryptic fragments with either arginine (R) or lysine (K) residues at the carboxyl terminus.

BandIDCombined MASCOT scoreSequenceMASCOT scorem/z precursor ionCharge
10gi|62645718 similar to myosin-binding protein C (Rattus norvegicus)32(R)QAPPSEYER(I)32538.772
15KCRS_RAT creatine kinase221(R)GLKEVER(L)17415.752
(K)FEEILTR(L)32453.772
(K)LSVEALNSLTGEFK(G)56754.412
(R)LGYILTEPSNLGTGLR(A)40867.992
(R)LGSSEVEQVQLVVDGVK(L)96893.482
(R)EVENVAITALEGLKGDLAGR(Y)53685.713
(R)GTGGVDTAAVADVYDISNIDR(I)1061055.022
16G3P_RAT glyceraldehyde-3-phosphate dehydrogenase143(K)VGVNGFGR(I)44403.232
(−)VKVGVNGFGR(I)29516.812
(R)GAAQNLIPASTGAAK(A)34685.392
(K)LVINGKPITIFQER(D)36543.333
17TPIS_RAT triose-phosphate isomerase67(K)VVFEQTK(A)29425.742
(K)FFVGGNWK(M)11477.752
(K)CNVSEGVAQCTR(I)27690.822
19KCRS_RAT creatine kinase53(R)EVENVAITALEGLKGDLAGR(Y)53685.713
21MYH6_RAT myosin heavy chain326(R)INATLETK(Q)27445.262
(K)LQTENGELAR(Q)32565.802
(R)TKYETDAIQR(T)20612.832
(K)LASADIETYLLEK(S)75733.392
(R)IEELEEELEAER(T)37744.882
(R)ILNPAAIPEGQFIDSR(K)43870.982
(K)GTLEDQIIQANPALEAFGNAK(T)981100.562
22gi|62662968 similar to M-protein (R. norvegicus)166(R)ILTVEGLTEGSLYEFK(I)71899.982
(R)FLSELAQLEENVQLAR(T)97930.512
23MYPC3_MOUSE myosin-binding protein C304(K)LLCETEGR(V)28489.252
(K)QGVLTLEIR(K)56514.822
(R)NSPTDTILFIR(A)55638.872
(R)TSGGQALAELIVQEK(K)117772.422
(R)LEAPAEEDVWEILR(Q)51835.452
(K)VTEPAPPEK(A)21484.272
24KCRS_RAT creatine kinase157(K)VPPPLPQFGR(K)17554.332
(R)EVENVAITALEGLKGDLAGR(Y)43685.713
(R)GTGGVDTAAVADVYDISNIDR(I)991055.002
25gi|62663741 actinin α-2 (R. norvegicus)123(K)VIQSYSIR(I)24483.292
(R)LASELLEWIR(R)50615.362
(R)VGWELLLTTIAR(T)28686.412
(R)ILASDKPYILAEELR(R)28577.683
26ECHA_RAT trifunctional enzyme α subunit64(K)TGLEQGNDAGYLAESEKFGELALTK(E)64881.113
28DESM_RAT desmin42(R)TFGGAPFSLGSPLSSPVFPR(A)451039.572
29KCRS_RAT creatine kinase263(K)VPPPLPQFGR(K)18554.332
(R)EVENVAITALEGLK(G)41743.422
(R)LGYILTCPSNLGTGLR(A)40867.972
(R)EVENVAITALEGLKGDLAGR(Y)71685.723
(R)GTGGVDTAAVADVYDISNIDR(I)931055.012
30G3P_RAT glyceraldehyde-3-phosphate dehydrogenase34(R)GAAQNIIPASTGAAK(A)34685.402
33AAQ88168 Met-induced mitochondrial protein long (Homo sapiens)26(R)GLFTGLTPR(L)26481.282