Table I

Accession numbers for human proteins matches, expressed regions, and KD data for phosphopeptide binding clones

Since binding saturation was not reached at the highest concentration tested (20 μm), the affinity of SH2B and tensin clones for EGFRpY1173 was not determined (ND). No measurable binding to the non-phosphorylated peptides was observed. Accession numbers were obtained from the NCBI protein database. aa, amino acids.

ProteinAccession no.Expressed aaKD
EGFRpY1173FAKpY397
μm
APS 34098358 401–5271.5 ± 0.30.5 ± 0.1
PIK3R3 56205427 34–2483.3 ± 0.98.2 ± 1.8
SH2B 14715079 80–425ND1.9 ± 0.5
Tensin 17978514 1453–1574ND2.7 ± 0.2